Wu Yu-Lin, Huang Si-Han, He Chun-Mei, Qiu Bo, Liu Jing-Jing, Li Jia, Lin Ying, Yu Sheng-Lu, Wang Hong-Feng, Zhang Gui-Fang
Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, China.
Medical College of Jiaying University, Jiaying University, Meizhou 514000, China.
Evid Based Complement Alternat Med. 2020 Oct 14;2020:1421853. doi: 10.1155/2020/1421853. eCollection 2020.
The study aimed to evaluate the protective effect of flower extraction (DOFE) on alcohol-induced liver injury and its probable mechanisms in mice. The chemical composition of DOFE was performed via UPLC/MS. Male Kunming mice were used to establish alcohol-induced liver injury models by oral gavage of 56% alcohol. Results showed that DOFE dramatically attenuated the increased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), and triacylglycerol (TG). Meanwhile, hematoxylin and eosin and Oil Red O staining showed that DOFE attenuated degeneration, inflammatory infiltration, and lipid droplet accumulation. DOFE was also found to suppress the activity of malonaldehyde (MDA) and enhanced the level of glutathione (GSH) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the liver. The protection of DOFE against oxidative stress was associated with the downregulation of hepatic cytochrome P450 2E1 (CYP2E1) and upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H quinone oxidoreductase l (NQO1). Additionally, DOFE suppressed inflammation via downregulating Toll-like receptor-4 (TLR-4) and nuclear factor kappa-B P65 (NF-B P65). Thus, DOFE exhibited a significant protective effect against alcohol-induced liver injury through its antisteatosis, antioxidative, and anti-inflammatory effect.
本研究旨在评估花朵提取物（DOFE）对小鼠酒精性肝损伤的保护作用及其可能机制。通过超高效液相色谱/质谱联用仪（UPLC/MS）对DOFE的化学成分进行分析。选用雄性昆明小鼠，通过灌胃56%酒精建立酒精性肝损伤模型。结果显示，DOFE显著降低了血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、总胆固醇（TC）和甘油三酯（TG）水平的升高。同时，苏木精-伊红染色和油红O染色显示，DOFE减轻了肝组织的变性、炎症浸润和脂滴蓄积。研究还发现，DOFE可抑制肝脏中丙二醛（MDA）的活性，提高谷胱甘肽（GSH）水平以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的活性。DOFE对氧化应激的保护作用与肝脏细胞色素P450 2E1（CYP2E1）的下调以及核因子红细胞2相关因子2（Nrf2）、血红素加氧酶-1（HO-1）和NAD（P）H醌氧化还原酶1（NQO1）的上调有关。此外，DOFE通过下调Toll样受体4（TLR-4）和核因子κB P65（NF-κB P65）来抑制炎症反应。因此，DOFE通过其抗脂肪变性、抗氧化和抗炎作用，对酒精性肝损伤表现出显著的保护作用。
