INSERM, Unité Mixte de Recherche UMR 1109, Team "Osteoarticular and Dental Regenerative NanoMedicine," Fédération de Médecine Translationnelle de Strasbourg, Faculté de Chirurgie Dentaire, UDS, Strasbourg, France.
Faculty of Chemistry, Jagiellonian University, Kraków, Poland.
Nanomedicine. 2017 Oct;13(7):2231-2240. doi: 10.1016/j.nano.2017.06.007. Epub 2017 Jun 22.
Chitosan scaffolds of different deacetylation degrees, average molecular weights and concentrations reinforced with silica nanoparticles were prepared for bone tissue regeneration. The resulting nanocomposites showed similar pore sizes (<300 μm) regardless the deacetylation degree and concentration used in their formulation. Their mechanical compression resistance was increased by a 30% with the addition of silica nanoparticles as nanofillers. The biocompatibility of the three-dimensional chitosan scaffolds was confirmed by the Alamar Blue assay in human primary osteoblasts as well as the formation of cell spheroids indicative of their great potential for bone regeneration. In vivo implantation of the scaffolds in a mice calvaria defect model provided substantial evidences of the suitability of these nanocomposites for bone tissue engineering showing a mature and dense collagenous tissue with small foci of mineralization, vascularized areas and the infiltration of osteoblasts and osteoclasts. Nevertheless, mature bone tissue formation was not observed after eight weeks of implantation.
不同脱乙酰度、平均分子量和浓度的壳聚糖支架与硅纳米颗粒增强，用于骨组织再生。无论在其配方中使用的脱乙酰度和浓度如何，所得纳米复合材料都表现出相似的孔径（<300μm）。通过添加硅纳米颗粒作为纳米填料，其抗压强度提高了 30%。三维壳聚糖支架的生物相容性通过在人原代成骨细胞中的 Alamar Blue 测定以及细胞球状体的形成得到证实，这表明它们在骨再生方面具有巨大的潜力。在小鼠颅骨缺损模型中的支架植入提供了这些纳米复合材料适用于骨组织工程的大量证据，显示出成熟和密集的胶原组织，伴有少量矿化、血管化区域以及成骨细胞和破骨细胞的浸润。然而，植入八周后未观察到成熟的骨组织形成。
