河流型水牛（Bubalus bubalis）MDGI基因的PCR-SSCP分析及其与产奶性状的关联
PCR-SSCP analysis of MDGI gene and its association with milk production traits in river buffalo (Bubalus bubalis).
作者信息
Dubey P K, Dubey S, Mishra S K, Arora R, Patel J, Singh K P, Kathiravan P, Mishra B P, Kataria R S
机构信息
National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India.
National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India.
出版信息
Res Vet Sci. 2017 Dec;115:307-309. doi: 10.1016/j.rvsc.2017.06.006. Epub 2017 Jun 12.
In this study, we investigated the genetic variation within 3'UTR of Mammary-Derived Growth Inhibitor (MDGI) gene of buffalo using PCR-SSCP and sequencing; and also analyzed association of polymorphism with the milk production traits. The study revealed two conformational patterns, 'A' and 'B' among 234 Mehsana buffaloes maintained with their records in the field and at farm. The frequency of SSCP variant 'A' was found to be invariably high in the buffalo population under study. Further, association analysis of SSCP variants with various milk production and milk quality traits indicated no significant effect on any of the traits investigated. Sequencing of SSCP variant 'A' showed homozygous G/G and A/A and 'B' had heterozygous G/C and A/G at positions +124 and +140 respectively, in the 3'UTR of buffalo MDGI. The preliminary results showed the substantial variations in the distribution of SSCP variants' frequencies within Mehsana buffaloes, however these variants had non-significant association with milk yield, fat yield and fat percentage in Mehsana buffaloes.
在本研究中，我们利用聚合酶链反应-单链构象多态性（PCR-SSCP）和测序技术，对水牛乳腺衍生生长抑制因子（MDGI）基因3'非翻译区（3'UTR）内的遗传变异进行了研究；并分析了多态性与产奶性状之间的关联。该研究在234头在实地和农场记录了相关信息的梅萨纳水牛中发现了两种构象模式，即“A”和“B”。在所研究的水牛群体中，SSCP变异体“A”的频率始终较高。此外，对SSCP变异体与各种产奶量和乳品质性状的关联分析表明，其对所研究的任何性状均无显著影响。对SSCP变异体“A”进行测序发现，在水牛MDGI基因的3'UTR中，+124和+140位点分别为纯合的G/G和A/A，而变异体“B”为杂合的G/C和A/G。初步结果表明，梅萨纳水牛中SSCP变异体频率的分布存在显著差异，然而这些变异体与梅萨纳水牛的产奶量、乳脂肪产量和乳脂肪率无显著关联。
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