卵泡抑素在乳腺癌中表达增加可降低侵袭性，并在临床上与更好的生存率相关。
Increased Expression of Follistatin in Breast Cancer Reduces Invasiveness and Clinically Correlates with Better Survival.
作者信息
Zabkiewicz Catherine, Resaul Jeyna, Hargest Rachel, Jiang Wen Guo, Ye Lin
机构信息
Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K.
出版信息
Cancer Genomics Proteomics. 2017 Jul-Aug;14(4):241-251. doi: 10.21873/cgp.20035.
Abstract
BACKGROUND/AIM: Activin and its antagonist follistatin (FST) have been implicated in several solid tumours. This study investigated the role of FST in breast cancer.
MATERIALS AND METHODS
FST expression was examined using reverse transcription polymerase chain reaction (RT-PCR), real-time quantitative polymerase chain reaction (qPCR) and immunohistochemistry in a cohort of breast cancer samples. Expression was correlated to pathological and prognostic parameters in our patient cohort. FST was overexpressed in MCF-7 cells and assays for growth and invasion were performed.
RESULTS
FST is expressed in breast tissue, in the cytoplasm of mammary epithelial cells. Expression was decreased in breast cancer tissue in comparison to normal mammary tissue. Over-expression of FST in vitro led to significantly increased growth rate and reduced invasion. Higher FST associates with lower-grade tumours and better survival.
CONCLUSION
Our results suggest a role for FST as a suppressor of invasion and metastasis in breast cancer.
摘要
背景/目的：激活素及其拮抗剂卵泡抑素（FST）与多种实体瘤有关。本研究调查了FST在乳腺癌中的作用。
材料与方法
使用逆转录聚合酶链反应（RT-PCR）、实时定量聚合酶链反应（qPCR）和免疫组织化学检测一组乳腺癌样本中的FST表达。将表达与我们患者队列中的病理和预后参数相关联。在MCF-7细胞中过表达FST，并进行生长和侵袭检测。
结果
FST在乳腺组织中表达，位于乳腺上皮细胞的细胞质中。与正常乳腺组织相比，乳腺癌组织中的表达降低。体外过表达FST导致生长速率显著增加和侵袭减少。较高的FST与较低级别的肿瘤和更好的生存率相关。
结论
我们的结果表明FST在乳腺癌中作为侵袭和转移的抑制剂发挥作用。
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