婴儿利什曼原虫无症状人类献血者携带者：伊朗西北部内脏利什曼病的潜在储存宿主。

Asymptomatic human blood donors carriers of Leishmania infantum: Potential reservoirs for visceral leishmaniasis in northwestern Iran.

作者信息

Asfaram Shabnam, Fakhar Mahdi, Mohebali Mehdi, Mardani Ahmad, Banimostafavi Elham Sadat, Ziaei Hezarjaribi Hajar, Soosaraei Masoud

机构信息

Student Research Committee, Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Molecular and Cell Biology Research Center, Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Transfus Apher Sci. 2017 Jun;56(3):474-479. doi: 10.1016/j.transci.2017.06.001. Epub 2017 Jun 9.

DOI:10.1016/j.transci.2017.06.001

PMID:28648574

Abstract

BACKGROUND

Little is known regarding transfusion-transmitted leishmaniasis (TTL) and the real global incidence of visceral leishmaniasis (VL) among blood donors as carriers of Leishmania spp. in endemic VL foci including Iran. Recent epidemiological evidences indicate that blood donor may be harbor of Leishmania infantum (L. infantum) infection in Iran.

METHODS

The aim of the present study was to determine the prevalence of Leishmania infection among blood donors in a main endemic focus of VL in Iran using DAT and polymerase chain reaction (PCR) based methods Between July-September 2016, blood samples were collected from 600 apparently healthy blood donors from six blood donation centers and blood donation mobile unit in Ardabil Province, where VL cases had been recorded. Each of these samples was tested for anti-Leishmania antibodies, in direct agglutination test (DAT), and for L. infantum kDNA, the PCR-based assay.

RESULTS

Of 600 blood donors, which were examined, 23 (3.8%) blood donors were seropositive by DAT and of 23 seropositive subjects, 82.6% (19/23) were positive by PCR. All the seropositive cases were males except one of them was female.

CONCLUSIONS

Our findings showed that many asymptomatic human carriers of L. infantum live in the endemic regions of northwestern Iran and potentially act as reservoirs of infection; those must be considered carefully by arrangement VL control strategies in the country.

摘要

背景

关于输血传播的利什曼病（TTL）以及在包括伊朗在内的内脏利什曼病（VL）流行地区，献血者作为利什曼原虫属携带者时内脏利什曼病的实际全球发病率，人们了解甚少。最近的流行病学证据表明，伊朗的献血者可能是婴儿利什曼原虫（L. infantum）感染的携带者。

方法

本研究的目的是使用基于直接凝集试验（DAT）和聚合酶链反应（PCR）的方法，确定伊朗一个主要内脏利什曼病流行地区献血者中利什曼原虫感染的患病率。2016年7月至9月期间，从阿尔达比勒省六个献血中心和献血流动单位的600名表面健康的献血者中采集血样，该省曾记录到内脏利什曼病病例。对每个样本进行抗利什曼原虫抗体的直接凝集试验（DAT）检测以及基于PCR的婴儿利什曼原虫kDNA检测。