Rogers Loranzie S, Van Wert Jacey C, Mensinger Allen F
Marine Biological Laboratory, Woods Hole, MA 02543, United States; Department of Biology, University of Minnesota-Duluth, Duluth, MN 55812, United States.
Department of Biology, University of Minnesota-Duluth, Duluth, MN 55812, United States; University of California, Berkeley, Berkeley, CA, United States.
J Neurosci Methods. 2017 Aug 15;288:29-33. doi: 10.1016/j.jneumeth.2017.06.012. Epub 2017 Jun 22.
Chronically implanted electrodes allow monitoring neural activity from free moving animals. While a wide variety of implanted headstages, microdrives and electrodes exist for terrestrial animals, few have been developed for use with aquatic animals.
A two axis micromanipulator was fabricated with a Formlabs 3D printer for implanting electrodes into free-swimming oyster toadfish (Opsanus tau). The five piece manipulator consisted of a base, body, electrode holder, manual screw drive and locking nut. The manipulator measured approximately 25×20×30mm (l×w×h) and weighed 5.28g after hand assembly.
Microwire electrodes were inserted successfully with the manipulator to record high fidelity signals from the anterior lateral line nerve of the toadfish.
COMPARISON WITH EXISTING METHOD(S): The micromanipulator allowed the chronically implanted electrodes to be repositioned numerous times to record from multiple sites and extended successful recording time in the toadfish by several days.
Three dimensional printing allowed an inexpensive (<$US 5 material), two axis micromanipulator to be printed relatively rapidly (<2h) to successfully record from multiple sites in the anterior lateral line nerve of free-swimming toadfish.
长期植入的电极可用于监测自由活动动物的神经活动。虽然有各种各样的植入式前置放大器、微驱动器和电极用于陆生动物，但很少有专门为水生动物开发的。
使用Formlabs 3D打印机制造了一种双轴微操纵器，用于将电极植入自由游动的牡蛎蟾鱼（Opsanus tau）。这个由五部分组成的操纵器包括一个基座、主体、电极支架、手动螺杆驱动器和锁紧螺母。手工组装后，该操纵器尺寸约为25×20×30毫米（长×宽×高），重量为5.28克。
使用该操纵器成功插入微丝电极，以记录蟾鱼前侧线神经的高保真信号。
该微操纵器使长期植入的电极能够多次重新定位，以便从多个部位进行记录，并将蟾鱼的成功记录时间延长了几天。
三维打印能够相对快速地（<2小时）打印出一种低成本（材料成本<5美元）的双轴微操纵器，从而成功地从自由游动的蟾鱼前侧线神经的多个部位进行记录。
