Takeuchi Ichiro, Yamashiro Hideyuki, Gushi Mikako
Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Center of Advanced Technology for the Environment, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
MethodsX. 2019 Jun 13;6:1600-1607. doi: 10.1016/j.mex.2019.06.007. eCollection 2019.
The global decline of the tropical and subtropical coral reefs requires urgent completion of various experiments that will reveal the factors influencing coral health. We describe the procedure of a new inexpensive, easy, and fast method for attaching fragments of the hermatypic coral spp. to small polycarbonate hexagon head bolts using Bondic, an ultraviolet (UV)-curable oligomer-based adhesive agent made by Laser Bonding Tech, Inc. (Aurora, ON, Canada). The attachment was hardened within 10 s after applying the adhesive to the cut surface of the coral fragment. The corals attached to polycarbonate bolt were tolerant to long-distance aerial transport 3 days after the attachment. In addition to its implementation in various experiments using hermatypic corals, this method will contribute to aquaculture of hermatypic corals, exhibition of corals in aquariums, and coral reef restoration. The advantages of this new method are summarized below: •A new UV-curable oligomer-based adhesive agent is used as an artificial substrate for coral.•This method is inexpensive, easy to use, and coral attaches quickly to the artificial substrate.•Corals attached to the artificial substrate can withstand long periods of transportation.
热带和亚热带珊瑚礁在全球范围内的衰退，需要尽快完成各项实验，以揭示影响珊瑚健康的因素。我们描述了一种新的低成本、简便且快速的方法，该方法使用由激光粘结技术公司（加拿大安大略省奥罗拉市）生产的基于紫外线（UV）固化低聚物的粘合剂Bondic，将造礁珊瑚碎片附着到小型聚碳酸酯六角头螺栓上。在将粘合剂涂抹到珊瑚碎片的切割表面后，附着在10秒内就会硬化。附着在聚碳酸酯螺栓上的珊瑚在附着3天后能够耐受长途航空运输。除了在使用造礁珊瑚的各种实验中应用外，该方法还将有助于造礁珊瑚的水产养殖、珊瑚在水族馆中的展示以及珊瑚礁的恢复。这种新方法的优点总结如下：•一种基于紫外线固化低聚物的新型粘合剂被用作珊瑚的人工基质。•该方法成本低、易于使用，并且珊瑚能快速附着到人工基质上。•附着在人工基质上的珊瑚能够经受长时间运输。
