Li Ruirui, Duan Na, Zhang Yuanhui, Liu Zhidan, Li Baoming, Zhang Dongming, Dong Taili
Laboratory of Environment-Enhancing Energy (E2E) and Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China.
Laboratory of Environment-Enhancing Energy (E2E) and Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China.
Waste Manag. 2017 Oct;68:120-127. doi: 10.1016/j.wasman.2017.06.028. Epub 2017 Jun 22.
Anaerobic digestion (AD) is a promising alternative for livestock manure management. This paper presents the experimental results obtained through a batch experiment by using chicken manure (CM) and microalgae Chlorella sp. as co-substrates. The effect of co-digestion was evaluated by varying CM to Chlorella sp. ratios (0:10, 2:8, 4:6, 6:4, 8:2, 10: 0 based on the volatile solids (VS)). The major objective of this study is to evaluate the feasibility and synergistic impact of co-digestion of CM and Chlorella sp. Enhanced 14.20% and 76.86% methane production than CM and Chlorella sp. mono-digestion respectively was achieved in co-digestion at the ratio 8:2. In addition, the co-digestion at the ratio 8:2 showed significantly higher methane yield than the weighted average of the individual substrates' specific methane yield (WSMY), indicating strong synergy effect. The Illumina Miseq sequencing analysis showed that the AD process suppressed the acetoclastic methanogenesis Methanosaeta content; but partly enhanced hydrogenotrophic methanogenesis Methanosarcina, Methanospirillum and Methanobacterium, which was responsible for the methane production. The pre-treated microalgae was then introduced at the optimal ratio 8:2 to estimate the effect of pre-treatment of microalgae on AD process. However, the pre-treatment exhibited no positive effect.
厌氧消化（AD）是一种很有前景的家畜粪便管理替代方法。本文介绍了通过使用鸡粪（CM）和小球藻属微藻作为共底物进行批次实验所获得的实验结果。通过改变CM与小球藻属的比例（基于挥发性固体（VS）为0:10、2:8、4:6、6:4、8:2、10:0）来评估共消化的效果。本研究的主要目的是评估CM和小球藻属共消化的可行性和协同影响。在8:2的比例下进行共消化时，分别比CM和小球藻属单消化提高了14.20%和76.86%的甲烷产量。此外，8:2比例的共消化显示出比单个底物的比甲烷产量加权平均值（WSMY）显著更高的甲烷产率，表明有很强的协同效应。Illumina Miseq测序分析表明，AD过程抑制了乙酸裂解产甲烷菌Methanosaeta的含量；但部分增强了氢营养型产甲烷菌Methanosarcina、Methanospirillum和Methanobacterium，它们是产生甲烷的原因。然后以8:2的最佳比例引入预处理后的微藻，以评估微藻预处理对AD过程的影响。然而，预处理没有表现出积极效果。
