Riazuddin -, Khan Sarzamin, Imtiaz Naila, Aslam Saima, Ahmad Shakoor, Khan Hamayun, Rabbani Masood, Muhammad Javed, Tanveer Zafar Iqbal, Ali Sakhawat
Department of Poultry Science, The University of Agriculture, Peshawar, KPK, Pakistan.
Department of Animal Health, The University of Agriculture, Peshawar, KPK, Pakistan.
Pak J Pharm Sci. 2017 Mar;30(2):541-549.
The present study was conducted to investigate the quality and efficacy of commercially available preparations of tylosin and doxycycline available in the local market at Peshawar for poultry. In vitro and in vivo, tests were conducted to check the quality of these antimicrobial drugs. In vitro quality control test was performed by High performance liquid chromatographic (HPLC) and micro dilution method. In vivo, efficacy of the test drugs was checked in broilers infected with Mycoplasma gallisepticum. Results of HPLC indicated that test drug-2 contains doxycycline hydrochloride within specified limits but contain high quantity of active ingredient (Tylosin tartrate 120%). Recovery percentage of test drugs (3, 4, 5) were below the pharmacopoeial limit, which contained low quantity of tylosin tartrate (85%, 87.5%, 85%) respectively however, percent recovery of doxycycline were in the appropriate limits. All the tested drugs were effective against Mycoplasma gallisepticum and showed minimum inhibitory concentration (MIC) at 1.9μg/ml. The in vivo result indicated that all tested drugs decreased morbidity and mortality in infected chicks. The birds treated with test drugs (3 and 5) showed mortality of 9.5%, which was slightly higher than the other test groups. The current study suggested that there are incidences of substandard drugs in Pakistan and the drug regularity authorities should take strict actions against the manufacturing companies.
本研究旨在调查白沙瓦当地市场上家禽用泰乐菌素和强力霉素市售制剂的质量和疗效。进行了体外和体内试验以检查这些抗菌药物的质量。体外质量控制试验采用高效液相色谱法（HPLC）和微量稀释法进行。在体内，在感染鸡毒支原体的肉鸡中检查受试药物的疗效。HPLC结果表明，受试药物2中的盐酸强力霉素含量在规定限度内，但活性成分（酒石酸泰乐菌素）含量较高（120%）。受试药物3、4、5的回收率低于药典限度，其酒石酸泰乐菌素含量分别较低（85%、87.5%、85%），不过强力霉素的回收率在适当限度内。所有受试药物对鸡毒支原体均有效，最低抑菌浓度（MIC）为1.9μg/ml。体内结果表明，所有受试药物均降低了感染雏鸡的发病率和死亡率。用受试药物3和5治疗的鸡死亡率为9.5%，略高于其他试验组。当前研究表明，巴基斯坦存在不合格药品的情况，药品监管当局应对生产公司采取严厉行动。
