Faculty of Pharmacy, Cairo University, Egypt.
Faculty of Pharmacy, Ahram Canadian University, Egypt.
J Food Sci. 2021 Mar;86(3):1162-1171. doi: 10.1111/1750-3841.15640. Epub 2021 Feb 18.
Residues of veterinary drugs in poultry meat have serious health effects on humans (e.g., antimicrobial resistance, carcinogenicity, and hypersensitivity), which make the control of veterinary drug residues an important parameter in ensuring consumer protection. This work was performed to quantitatively determine two co-formulated anti-infective veterinary agents, tylosin tartrate (TYT) and doxycycline hydrochloride (DOX) in different tissues of broiler chickens (liver, muscles, and fat) using high performance liquid chromatography. The chicken was treated with the recommended dose of a binary mixture of the drugs (Tydovet). Moreover, the study aimed to estimate the withdrawal time of both drugs in chicken tissues. The analysis was done by solvent extraction and solid-phase extraction for clean-up of samples from the tissue matrix, followed by liquid chromatographic determination of the cited drugs with UV-detection. Residue decline with time was tracked, and both antibiotics were found to be more persistent in liver tissues than other tissues (muscle and fat). The effect of freezing and cooking was investigated on tissue residue levels. While freezing had little effect on the concentration of both antibiotics; cooking, as anticipated, led to a marked decline. Therefore, it is recommended to pay attention to the proper withdrawal periods before marketing to ensure the hygienic suitability of broilers edibles for safe human consumption. PRACTICAL APPLICATION: This novel study measures tylosin and doxycycline residues simultaneously in different tissues (muscle, fat, and liver) after administration of Tydovet powder to the broiler chicken. Residues in fat persisted for a longer time than in muscle in case of TYT, whereas the reverse was noticed in DOX.
兽用药物残留对人类健康有严重影响（例如，抗药性、致癌性和过敏反应），这使得控制兽药残留成为保障消费者安全的一个重要参数。本工作采用高效液相色谱法，定量测定了肉鸡（肝、肌肉和脂肪）不同组织中两种复方抗感染兽用药物酒石酸泰乐菌素（TYT）和盐酸多西环素（DOX）的残留量。鸡用推荐剂量的二元混合物（Tydovet）进行处理。此外，本研究旨在估计两种药物在鸡组织中的停药时间。分析采用溶剂萃取和固相萃取进行样品净化，从组织基质中提取，然后用紫外检测进行液相反相色谱测定。跟踪了随时间的残留减少情况，发现两种抗生素在肝组织中的残留时间比其他组织（肌肉和脂肪）长。还研究了冷冻和烹饪对组织残留水平的影响。虽然冷冻对两种抗生素的浓度影响不大，但如预期的那样，烹饪导致残留显著下降。因此，建议在上市前注意适当的停药期，以确保肉鸡可食用部分的卫生适宜性，确保人类安全食用。实际应用：本研究新颖之处在于，在给肉鸡服用 Tydovet 粉后，同时测量了不同组织（肌肉、脂肪和肝脏）中泰乐菌素和多西环素的残留量。在 TYT 的情况下，脂肪中的残留时间比肌肉中的残留时间长，而 DOX 则相反。
