Singh Anita
a Department of Biomedical Engineering , Widener University , Chester , PA , USA.
Brain Inj. 2017;31(10):1387-1395. doi: 10.1080/02699052.2017.1321781. Epub 2017 Jun 26.
Secondary axotomy is more prevalent than the primary axotomy and involves subtle intraaxonal changes in response to the injury leading to cytoskeletal disruptions including neurofilament (NF) misalignment and compaction, which is associated with the genesis of impaired axoplasmic transport (IAT). Recent studies have reported two differential axonal responses to injury, one associated with the cytoskeletal collapse and another with the IAT. The objective of this study was to determine the extent of IAT and early NF changes in axons that were subjected to a stretch of various degrees at different strain rates.
Fifty-six L5 dorsal spinal nerve roots were subjected to a predetermined strain at a specified displacement rate (0.01 and 15 mm/second) only once. The histological changes were determined by performing standard immunohistochemical procedures using beta amyloid precursor protein (β APP) and NF-68 kDa antibodies.
No significant differences in the occurrence rate of either of the staining in the axons were observed when subjected to similar loading conditions, and the occurrence rate of both β APP and NF68 staining was strain and rate-dependent.
继发性轴突切断比原发性轴突切断更常见，它涉及轴突内对损伤的细微变化，导致细胞骨架破坏，包括神经丝（NF）排列紊乱和压缩，这与轴浆运输受损（IAT）的发生有关。最近的研究报道了轴突对损伤的两种不同反应，一种与细胞骨架塌陷有关，另一种与IAT有关。本研究的目的是确定在不同应变率下受到不同程度拉伸的轴突中IAT的程度和早期NF变化。
56条L5背侧脊神经根仅以预定的位移速率（0.01和15毫米/秒）施加一次预定应变。通过使用β淀粉样前体蛋白（β APP）和NF-68 kDa抗体进行标准免疫组织化学程序来确定组织学变化。
在相似的加载条件下，轴突中任何一种染色的发生率均未观察到显著差异，并且β APP和NF68染色的发生率均与应变和速率相关。
