Buldur Burak, Kapdan Arife
J Clin Pediatr Dent. 2017;41(4):284-288. doi: 10.17796/1053-4628-41.4.284.
The purpose of this study was to compare the antimicrobial efficacy of the EndoVac system and conventional needle irrigation to eliminate E faecalis in primary molar root canals.
60 extracted human primary second molar roots were instrumented up to an apical size .04/35 and randomly divided into two groups; Group 1: conventional needle (n=30) and Group 2: EndoVac (n=30), and four subgroups (two experimental subgroups; (a) 2.5% sodium hypochlorite (NaOCl) + ethylenediaminetetraacetic acid (EDTA) (n=20), (b) ozonated water (OW) + EDTA (n=20), and control groups (c) 5.25% NaOCl (n=10) and (d) saline (n=10). All roots were sterilized and then inoculated with E.faecalis. Before and after final irrigation procedures, root canals were sampled and the grown colony forming units (CFUs) were counted. Data were analyzed by Kruskall-Wallis and Mann-Whitney U tests using a 0.05 significance level.
The EndoVac reduced more bacteria than the conventional needle did but it was not statistically significant (p>0.05). NaOCl alone or followed by EDTA totally eliminated bacteria. OW + EDTA showed higher reduction of bacteria but could not totally eliminate bacterias.
In the context of bacterial elimination, the EndoVac was not significantly better than the conventional needle. Although, there were fewer CFU/mg when using EndoVac, there was not any statistically significant superiority to conventional needle irrigation. An OW+EDTA regimen showed antibacterial effect in the primary molar root canals but it was significantly less effective than NaOCl+EDTA.
本研究旨在比较EndoVac系统与传统针管冲洗在消除乳磨牙根管内粪肠球菌方面的抗菌效果。
选取60颗拔除的人乳磨牙牙根,预备至根尖尺寸0.04/35,随机分为两组;第1组:传统针管冲洗组(n = 30),第2组:EndoVac组(n = 30),并进一步分为四个亚组(两个实验组;(a)2.5%次氯酸钠(NaOCl)+乙二胺四乙酸(EDTA)(n = 20),(b)臭氧水(OW)+ EDTA(n = 20),以及对照组(c)5.25% NaOCl(n = 10)和(d)生理盐水(n = 10)。所有牙根均进行消毒,然后接种粪肠球菌。在最终冲洗程序前后,对根管进行取样并计数生长的菌落形成单位(CFU)。采用Kruskal-Wallis检验和Mann-Whitney U检验对数据进行分析,显著性水平设定为0.05。
EndoVac比传统针管冲洗减少的细菌更多,但差异无统计学意义(p>0.05)。单独使用NaOCl或随后使用EDTA可完全消除细菌。OW + EDTA显示出较高的细菌减少率,但不能完全消除细菌。
在细菌消除方面,EndoVac并不显著优于传统针管冲洗。尽管使用EndoVac时每毫克CFU较少,但与传统针管冲洗相比,并无统计学上的显著优势。OW + EDTA方案在乳磨牙根管中显示出抗菌效果,但明显不如NaOCl + EDTA有效。