Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Int Endod J. 2013 Jun;46(6):499-505. doi: 10.1111/iej.12016. Epub 2012 Nov 9.
To evaluate the ex vivo efficacy of the EndoVac system and photodynamic treatment (PDT) as adjuncts to chemomechanical debridement associated with calcium hydroxide (CaOH2 ) in reducing the levels of intracanal Enterococcus faecalis.
One hundred and twenty-five sterile premolar teeth were conventionally accessed, prepared and then contaminated with E. faecalis (ATCC 29212) for 30 days. Teeth were randomly divided into 4 groups: Control (chemomechanical debridement with conventional irrigation); Endovac (chemomechanical debridement with EndoVac system); PDT (chemomechanical debridement with conventional irrigation and PDT) and Endovac+PDT (chemomechanical debridement with EndoVac and PDT). The irrigants used in all groups were 5.25% sodium hypochlorite and 17% EDTA. After treatment, an intracanal dressing (CaOH2 ) was applied in all canals for 7 days. Samples were obtained before (T1) and after the therapeutic procedures (T2) and, after intracanal medication (T3), plated onto BHI media and incubated (37 °C, 48 h) to determine the colony-forming units (CFU mL(-1) ).
The overall mean cell counts (CFU mL(-1) ) of E. faecalis were high at the initial contamination (T1). A significant reduction (P < 0.05) of E. faecalis mean counts was observed in all groups from baseline (T1) to both post-therapy samplings (T2 and T3); no differences amongst the groups were detected. No significant change in bacterial counts from T2 to T3 was detected.
The adjunctive use of the EndoVac system and the photodynamic treatment, in combination or not, was as effective as the conventional chemomechanical debridement associated with CaOH2 on reducing the counts of intracanal E. faecalis.
评估 EndoVac 系统和光动力疗法（PDT）作为化学机械清创术（辅以氢氧化钙 [CaOH2]）的辅助手段，以降低根管内粪肠球菌水平的体外疗效。
125 颗无菌前磨牙经常规开髓、预备，然后用粪肠球菌（ATCC 29212）污染 30 天。将牙齿随机分为 4 组：对照组（常规冲洗化学机械清创术）；Endovac 组（EndoVac 系统化学机械清创术）；PDT 组（常规冲洗化学机械清创术联合 PDT）和 Endovac+PDT 组（EndoVac 和 PDT 联合化学机械清创术）。所有组均使用 5.25%次氯酸钠和 17% EDTA 冲洗。所有根管均在治疗后应用根管内封药（CaOH2），共 7 天。分别于治疗前（T1）、治疗后（T2）和根管用药后（T3）采集样本，接种到 BHI 培养基中，在 37°C 孵育（48 h），以确定菌落形成单位（CFU·mL-1）。
粪肠球菌的总体平均细胞计数（CFU·mL-1）在初始污染时（T1）较高。所有组在基线（T1）至治疗后两次采样（T2 和 T3）时，粪肠球菌的平均计数均显著降低（P < 0.05）；各组间无差异。从 T2 到 T3，细菌计数无显著变化。
与常规的氢氧化钙化学机械清创术联合使用 EndoVac 系统和光动力疗法，联合或不联合使用，均能有效降低根管内粪肠球菌的数量。
