Centro de Química de Vila Real, Departamento de Química, UTAD - Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801, Vila Real, Portugal; Department of Chemical and Environmental Technology, ESCET, Universidad Rey Juan Carlos, C/ Tulipán s/n, 28933, Móstoles, Madrid, Spain.
Centro de Investigação e de Tecnologias Agroambientais e Biológicas (CITAB), Departamento de Biologia e Ambiente, UTAD, Quinta de Prados, 5000-801, Vila Real, Portugal.
Water Res. 2017 Oct 15;123:113-123. doi: 10.1016/j.watres.2017.06.021. Epub 2017 Jun 10.
Freshwater disinfection using photolytic and catalytic activation of peroxymonosulphate (PMS) through PMS/UV-A LED and PMS/M/UV-A LED [M = Fe or Co] processes was evaluated through the inactivation of three different bacteria: Escherichia coli (Gram-negative), Bacillus mycoides (sporulated Gram-positive), Staphylococcus aureus (non-sporulated Gram-positive), and the fungus Candida albicans. Photolytic and catalytic activation of PMS were effective in the total inactivation of the bacteria using 0.1 mM of PMS and M at neutral pH (6.5), with E. coli reaching the highest and the fastest inactivation yield, followed by S. aureus and B. mycoides. With B. mycoides, the oxidative stress generated through the complexity of PMS/M/UV-A LED combined treatments triggered the formation of endospores. The treatment processes were also effective in the total inactivation of C. albicans, although, due to the ultrastructure, biochemistry and physiology of this yeast, higher dosages of reagents (5 mM of PMS and 2.5 mM of M) were required. The rate of microbial inactivation markedly increased through catalytic activation of PMS particularly during the first 60 s of treatment. Co was more effective than Fe to catalyse PMS decomposition to sulphate radicals for the inactivation of S. aureus and C. albicans. The inactivation of the four microorganisms was well represented by the Hom model. The Biphasic and the Double Weibull models, which are based on the existence of two microbial sub-populations exhibiting different resistance to the treatments, also fitted the experimental results of photolytic activation of PMS.
采用 PMS/UV-A LED 和 PMS/M/UV-A LED [M=Fe 或 Co] 工艺通过光解和催化激活过一硫酸盐（PMS）对淡水进行消毒，通过对三种不同细菌（大肠杆菌（革兰氏阴性）、草分枝杆菌（有芽孢的革兰氏阳性）、金黄色葡萄球菌（无芽孢的革兰氏阳性）和真菌白色念珠菌）的失活来评估该工艺。在中性 pH（6.5）下，使用 0.1mM 的 PMS 和 M 时，光解和催化激活 PMS 可有效完全灭活细菌，其中大肠杆菌的失活率最高且最快，其次是金黄色葡萄球菌和草分枝杆菌。对于草分枝杆菌，通过 PMS/M/UV-A LED 联合处理产生的复杂氧化应激触发了芽孢的形成。该处理工艺也可有效完全灭活白色念珠菌，尽管由于该酵母的超微结构、生物化学和生理学特性，需要更高剂量的试剂（5mM 的 PMS 和 2.5mM 的 M）。通过催化激活 PMS，微生物的失活率在处理的最初 60 秒内显著提高。与 Fe 相比，Co 更有利于催化 PMS 分解为硫酸根自由基，从而灭活金黄色葡萄球菌和白色念珠菌。4 种微生物的失活均可以用 Hom 模型很好地表示。基于存在对处理具有不同抗性的两种微生物亚群的假设，双相和双 Weibull 模型也拟合了 PMS 光解的实验结果。
