Lu Qin-Kang, Zhang Jun-Tao, Zhao Na, Wang Hui-Yun, Tong Qi-Hu, Wang Shou-Li
The Eye Hospital of Wenzhou Medical University (Ningbo Branch); and Department of Ophthalmology, Yinzhou Hospital Affiliated to Medical School of Ningbo University, Zhejiang, China.
Ophthalmic Res. 2017;58(3):162-167. doi: 10.1159/000475670. Epub 2017 Jun 27.
To investigate the association of interleukin (IL)-6 with proliferative diabetic retinopathy (PDR) of type 2 diabetes (T2D) in a Chinese population.
Two subtypes of the IL-6 promoter (-174 and -572 G/C) were genotyped in 215 T2D patients with PDR and 207 T2D patients with a normal retinal function (controls) using the PCR-RFLP method. The mRNA and protein expression of IL-6 was examined by real-time PCR.
T2D patients with PDR had a significantly higher frequency of IL-6 -174 GC (OR 0.58; 95% CI 0.34-0.99; p = 0.011) and IL-6 -572 GG (OR 0.53; 95% CI 0.24-1.14; p = 0.016) than T2D controls. The mRNA expressions of the rs1800795 GC and rs1800796 GG genotype were significantly increased compared to other cases (Fsig = 0.002, p = 0.001, respectively), followed by a relative increase in IL-6 in protein.
IL-6 genotypes of rs1800795 GC and rs1800796 GG might point to a relatively high risk for T2D patients suffering from PDR in a Chinese population and they were associated with elevation of IL-6 expression in both mRNA and protein.
研究白细胞介素（IL）-6与中国人群2型糖尿病（T2D）增殖性糖尿病视网膜病变（PDR）的相关性。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对215例患有PDR的T2D患者和207例视网膜功能正常的T2D患者（对照组）进行IL-6启动子的两种亚型（-174和-572 G/C）基因分型。通过实时聚合酶链反应检测IL-6的mRNA和蛋白表达。
患有PDR的T2D患者中，IL-6 -174 GC（比值比0.58；95%置信区间0.34-0.99；p = 0.011）和IL-6 -572 GG（比值比0.53；95%置信区间0.24-1.14；p = 0.016）的频率显著高于T2D对照组。与其他病例相比rs1800795 GC和rs1800796 GG基因型的mRNA表达显著增加（F值分别为0.002和0.001，p值分别为0.001），随后IL-6蛋白相对增加。
rs1800795 GC和rs1800796 GG的IL-6基因型可能表明中国人群中患有PDR的T2D患者风险相对较高，并且它们与IL-6 mRNA和蛋白表达升高有关。
