Bloancă Vlad, Ceauşu Amalia Raluca, Jitariu Andreea Adriana, Barmayoun Ariana, Moş Raluca, Crăiniceanu Zorin, Bratu Tiberiu
Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania.
Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania.
In Vivo. 2017 Jul-Aug;31(4):649-655. doi: 10.21873/invivo.11107.
The aim of the study was to assess the effect of autologous fat graft on nerve regeneration by means of immunohistochemistry.
The rat sciatic nerve was used; complete transection followed by primary neurorrhaphy was performed on both hind legs, on the left side a processed fat graft was applied, surrounding the nerve. Nerve biopsies were collected and immunohistochemical procedures were performed for glial fibrillary acidic protein (GFAP) and for neurofilament-associated protein(NFAP).
At 4 weeks, GFAP-positive cells were observed in the connective tissue formed between the two nerve endings on the left side only. At 10 weeks, GFAP-positive structures were present and exhibited a tendency to become linear on both sides, with an increased density on the left. NFAP-positive expression was present in the left treated limb with a disorganized pattern.
Adipose tissue led to the stimulation of GFAP-positive Schwann cells, which could have a positive impact on nerve regeneration in the clinical setting.
本研究旨在通过免疫组织化学方法评估自体脂肪移植对神经再生的影响。
使用大鼠坐骨神经；对双侧后腿进行完全横断并一期神经缝合，左侧在神经周围应用经处理的脂肪移植物。采集神经活检标本，并对胶质纤维酸性蛋白（GFAP）和神经丝相关蛋白（NFAP）进行免疫组织化学检测。
4周时，仅在左侧两个神经断端之间形成的结缔组织中观察到GFAP阳性细胞。10周时，两侧均有GFAP阳性结构，且呈线性趋势，左侧密度增加。左侧治疗肢体中存在NFAP阳性表达，呈紊乱模式。
脂肪组织可刺激GFAP阳性的雪旺细胞，这可能对临床环境中的神经再生产生积极影响。
