Zhang Y, Kang Y, Qin Y, Zhou Z, Lei M, Guo H
Shaanxi Research Center of TCM Fingerprint and NP Library, College of Life Sciences, Northwest A&F University, Yangling, P.R. China.
Genet Mol Res. 2012 Dec 3;11(4):4121-9. doi: 10.4238/2012.December.3.1.
Polyporus umbellatus (Pers.) Fries is an endangered medicinal fungus in China with in vivo anticancer activity, but its genetic information is lacking. Eight natural P. umbellatus strains collected from 7 provinces in China were subjected to sequence-related amplified polymorphism markers to estimate the level and pattern of genetic diversity. Forty-nine primer combinations generated 1219 highly reproducible and discernible loci, among which 1023 were polymorphic. The percentage of polymorphism varied from 35.71 to 96.30 with an average of 83.92. Genetic identity among all strains ranged from 0.15 to 0.78 with an average of 0.46. The unweighted pair group method with arithmetic mean dendrogram clustered 8 strains into 3 clusters, and the clustering pattern showed 3 groups. Principal coordinate analysis further indicated that the genetic diversity of P. umbellatus strains was unevenly distributed and instead displayed a clustered distribution pattern. A relatively high level of genetic diversity was maintained in 8 natural P. umbellatus strains, but its abundance might be subjected to environmental heterogeneity, and the population structure of co-evolved Armillaria species may be selected by nature under the specific microenvironment.
猪苓（Polyporus umbellatus (Pers.) Fries）是中国一种具有体内抗癌活性的濒危药用真菌，但缺乏其遗传信息。对从中国7个省份采集的8株天然猪苓菌株进行了序列相关扩增多态性标记分析，以评估遗传多样性水平和模式。49对引物组合产生了1219个高度可重复且可分辨的位点，其中1023个为多态性位点。多态性百分比在35.71%至96.30%之间，平均为83.92%。所有菌株之间的遗传一致性在0.15至0.78之间，平均为0.46。采用算术平均法的非加权配对组方法构建的树状图将8个菌株聚为3个簇，聚类模式呈现出3个组。主坐标分析进一步表明，猪苓菌株的遗传多样性分布不均衡，而是呈现出聚类分布模式。8株天然猪苓菌株维持了相对较高水平的遗传多样性，但其丰富度可能受到环境异质性的影响，并且在特定微环境下，共同进化的蜜环菌物种的种群结构可能会受到自然选择。
