ImmunoPET Imaging of αβ Expression Using an Engineered Anti-αβ Cys-diabody Site-Specifically Radiolabeled with Cu-64: Considerations for Optimal Imaging with Antibody Fragments.

PURPOSE

Increased expression of the αβ integrin correlates with advanced tumor grade and poor clinical outcome, identifying αβ as a prognostic indicator and an attractive target for molecular imaging. This work investigated the ability of a disulfide-stabilized [Cu]NOTA-αβ cys-diabody to image αβ expression in vivo using a nu/nu mouse model bearing human melanoma xenografts and positron-emission tomography.

PROCEDURES

Small-animal positron emission tomography (PET) imaging, quantitative ROI analysis, and ex vivo biodistribution were conducted to ascertain tumor uptake and organ distribution of the [Cu]NOTA-αβ cys-diabody. Immunohistochemical staining of tumors and mouse organs and immunoreactivity assays were utilized to correlate in vivo and ex vivo observations.

RESULTS

PET imaging of the [Cu]NOTA-αβ cys-diabody revealed low tumor uptake at 24 h p.i. in DX3Puroβ tumors (2.69 ± 0.45 %ID/g) with comparable results found in the DX3Puro tumors (2.24 ± 0.15 %ID/g). Quantitative biodistribution confirmed that DX3Puroβ tumor uptake was highest at 24 h p.i. (4.63 ± 0.18 %ID/g); however, uptake was also observed in the stomach (4.84 ± 2.99 %ID/g), small intestines (4.50 ± 1.69 %ID/g), large intestines (4.73 ± 0.97 %ID/g), gallbladder (6.04 ± 1.88 %ID/g), and lungs (3.89 ± 0.69 %ID/g).

CONCLUSIONS

Small-animal PET imaging was successful in visualizing αβ-positive tumor uptake of the [Cu]NOTA-αβ cys-diabody. Cys-diabody cross-reactivity was observed between human and murine αβ and immunohistochemical staining confirmed the presence of an endogenous αβ antigen sink, which led to suboptimal tumor contrast in this mouse model. Future investigations will focus on dose escalation studies to overcome the endogenous antigen sink while increasing DX3Puroβ tumor uptake.