微粒体氧化在大鼠脑和肝脏中单胺氧化酶活性调节中的作用。

The role of microsomal oxidation in the regulation of monoamine oxidase activity in the brain and liver of rats.

作者信息

Kozochkin Denis A, Manukhina Eugenia B, Downey H Fred, Tseilikman Olga B, Komelkova Maria V, Vasilyeva Maria V, Lapshin Maxim S, Sahabutdinov Marat N, Lazuko Svetlana S, Tseilikman Vadim E

机构信息

South Ural State University Biomedical School, Chelyabinsk, Russia.

出版信息

Gen Physiol Biophys. 2017 Oct;36(4):455-464. doi: 10.4149/gpb_2017012. Epub 2017 Jun 27.

DOI:10.4149/gpb_2017012

PMID:28653655

Abstract

It has been shown in our previous study that monoamine oxidase (MAO) activity in different brain regions are correlated with a microsomal oxidation phenotype. The data obtained in this study, using the microsomal oxidation inhibitor SKF525, and using animals with different duration of hexobarbital sleep, has shown that increased intensity of microsomal oxidation might be associated with increased MAO activity. Since the rats with short hexobarbital sleep time had higher content of hepatic microsomal cytochrome P450 than did rats with long hexobarbital sleep time. In addition, the rats with higher hepatic content of CYP450 had higher activities of MAO-A and MAO-B. Moreover, the microsomal oxidation inhibitor SKF-525 reduced brain and liver activities of MAOA and MAO-B. Consequently, MAO activities in a brain and a liver depend on the microsomal oxidation process.

摘要

我们之前的研究表明，不同脑区的单胺氧化酶（MAO）活性与微粒体氧化表型相关。本研究使用微粒体氧化抑制剂SKF525，并对不同戊巴比妥睡眠时间的动物进行研究，结果表明微粒体氧化强度增加可能与MAO活性增加有关。因为戊巴比妥睡眠时间短的大鼠肝脏微粒体细胞色素P450含量高于戊巴比妥睡眠时间长的大鼠。此外，肝脏CYP450含量较高的大鼠MAO-A和MAO-B活性也较高。而且，微粒体氧化抑制剂SKF-525降低了脑和肝脏中MAO-A和MAO-B的活性。因此，脑和肝脏中的MAO活性取决于微粒体氧化过程。