Karam Amanda L, McMillan Catherine C, Lai Yi-Chun, de Los Reyes Francis L, Sederoff Heike W, Grunden Amy M, Ranjithan Ranji S, Levis James W, Ducoste Joel J
Department of Civil, Construction, and Environmental Engineering, North Carolina State University.
Department of Civil, Construction, and Environmental Engineering, North Carolina State University;
J Vis Exp. 2017 Jun 14(124):55545. doi: 10.3791/55545.
The optimal design and operation of photosynthetic bioreactors (PBRs) for microalgal cultivation is essential for improving the environmental and economic performance of microalgae-based biofuel production. Models that estimate microalgal growth under different conditions can help to optimize PBR design and operation. To be effective, the growth parameters used in these models must be accurately determined. Algal growth experiments are often constrained by the dynamic nature of the culture environment, and control systems are needed to accurately determine the kinetic parameters. The first step in setting up a controlled batch experiment is live data acquisition and monitoring. This protocol outlines a process for the assembly and operation of a bench-scale photosynthetic bioreactor that can be used to conduct microalgal growth experiments. This protocol describes how to size and assemble a flat-plate, bench-scale PBR from acrylic. It also details how to configure a PBR with continuous pH, light, and temperature monitoring using a data acquisition and control unit, analog sensors, and open-source data acquisition software.
用于微藻培养的光合生物反应器（PBR）的优化设计和运行对于提高基于微藻的生物燃料生产的环境和经济性能至关重要。估计不同条件下微藻生长的模型有助于优化PBR的设计和运行。为了有效，这些模型中使用的生长参数必须准确确定。藻类生长实验常常受到培养环境动态性质的限制，需要控制系统来准确确定动力学参数。建立受控批次实验的第一步是实时数据采集和监测。本方案概述了一个台式光合生物反应器的组装和操作过程，该反应器可用于进行微藻生长实验。本方案描述了如何用丙烯酸制作并组装一个平板台式PBR。它还详细说明了如何使用数据采集与控制单元、模拟传感器和开源数据采集软件配置一个具有连续pH、光照和温度监测功能的PBR。
