School of Mathematical and Statistical Sciences, Arizona State University, Tempe, AZ 85287, United States.
Bioresour Technol. 2011 Jan;102(1):111-7. doi: 10.1016/j.biortech.2010.06.029. Epub 2010 Jul 8.
Many green microalgae significantly increased their cellular neutral lipid content when cultured in nitrogen limited or high light conditions. Due to their lipid production potential, these algae have been suggested as promising feedstocks for biofuel production. However, no models for algal lipid synthesis with respect to nutrient and light have been developed to predict lipid production and to help improve the production process. A mathematical model is derived describing the growth dynamics and neutral lipid production of green microalgae grown in batch cultures. The model assumed that as the nitrogen was depleted, photosynthesis became uncoupled from growth, resulting in the synthesis and accumulation of neutral lipids. Simulation results were compared with experimental data for the green microalgae Pseudochlorococcum sp. For growth media with low nitrogen concentration, the model agreed closely with the data; however, with high nitrogen concentration the model overestimated the biomass. It is likely that additional limiting factors besides nitrogen could be responsible for this discrepancy.
许多绿色微藻在氮限制或高光条件下培养时，其细胞中性脂质含量显著增加。由于其产油潜力，这些藻类已被提议作为生物燃料生产的有前途的原料。然而，目前还没有针对营养和光照的藻类脂质合成模型来预测脂质产量并帮助改进生产工艺。本文推导了一个描述在分批培养中生长的绿色微藻的生长动态和中性脂质生产的数学模型。该模型假设，随着氮的耗尽，光合作用与生长脱耦，导致中性脂质的合成和积累。模拟结果与绿色微藻 Pseudochlorococcum sp 的实验数据进行了比较。对于氮浓度低的生长培养基，模型与数据吻合得很好；然而，对于高氮浓度，模型高估了生物量。除了氮之外，可能还有其他限制因素导致了这种差异。
