Mutti A, Lucertini S, Valcavi P, Neri T M, Fornari M, Alinovi R, Franchini I
Lancet. 1985 Oct 26;2(8461):914-7. doi: 10.1016/s0140-6736(85)90850-5.
Mouse monoclonal antibodies against brush-border antigens of the proximal tubule of human kidney were produced by the hybridisation technique. The urinary excretion of a brush-border protein with an apparent molecular weight of 50 000 (BB-50) was measured by a sandwich enzyme-linked immunosorbent assay with a mouse IgG1 against BB-50 and a polyclonal rabbit antiserum against human kidney as coating and second antibodies. The urinary excretion of BB-50 was fifty times higher in patients treated with cisplatin than in a matched control group and twice as high in workers occupationally exposed to water-soluble chromium(VI) compounds as in their matched controls. These findings suggest that the urinary excretion of kidney antigens revealed by monoclonal antibodies is a very sensitive and specific test for the assessment of toxic nephropathies.
采用杂交技术制备了抗人肾近端小管刷状缘抗原的小鼠单克隆抗体。通过夹心酶联免疫吸附测定法，以抗BB - 50的小鼠IgG1和抗人肾多克隆兔抗血清作为包被抗体和二抗，测定了表观分子量为50000的刷状缘蛋白（BB - 50）的尿排泄量。顺铂治疗患者的BB - 50尿排泄量比配对对照组高50倍，职业性接触水溶性六价铬化合物的工人的BB - 50尿排泄量是其配对对照组的两倍。这些发现表明，单克隆抗体检测出的肾抗原尿排泄量是评估中毒性肾病的一项非常敏感和特异的检测方法。
