Shu Yang, Liu Xue-Bing, Ma Xin-Hui, Gao Jian, He Wei, Cao Xian-Ying, Chen Jian
Department of Food Science, College of Food Science, Hainan University, Haikou 570100, China.
Department of Chemical Engineering and Technology, College of Materials and Chemical Engineering, Hainan University, Haikou 570100, China.
Environ Toxicol Pharmacol. 2017 Jul;53:191-198. doi: 10.1016/j.etap.2017.06.010. Epub 2017 Jun 20.
This study investigated the function of κ-carrageenan polysaccharide in immune regulation. The immune response of RAW 264.7 cells treated with κ-carrageenan polysaccharide was explored by MTT assay, general morphological observation, neutral red phagocytosis assay, Griess method, fluorescence method, and enzyme-linked immunosorbent assay. In addition, TLR4 blocking experiment and double-fluorescence immunostaining were performed on cells to demonstrate their immune response mechanism. Results show that κ-carrageenan polysaccharide not only promotes cell proliferation but also activates RAW 264.7 cells, thereby improving the cells' phagocytic capability, NO production, and tumor necrosis factor-α (TNF-α) secretion. In addition, the use of TLR4-specific inhibitors can significantly mediate the increased TNF-α secretion induced by κ-carrageenan polysaccharide. The RAW 264.7 cells treated with κ-carrageenan polysaccharide show upregulated TLR4 expression, and the main subunit of NF-κB (p65) is translocated. These results support the immunomodulatory function of κ-carrageenan polysaccharide in RAW 264.7 cells.
本研究调查了κ-卡拉胶多糖在免疫调节中的功能。通过MTT法、一般形态学观察、中性红吞噬试验、格里斯方法、荧光法和酶联免疫吸附试验，探究了用κ-卡拉胶多糖处理的RAW 264.7细胞的免疫反应。此外，对细胞进行了TLR4阻断实验和双荧光免疫染色，以证明其免疫反应机制。结果表明，κ-卡拉胶多糖不仅促进细胞增殖，还激活RAW 264.7细胞，从而提高细胞的吞噬能力、一氧化氮生成量和肿瘤坏死因子-α（TNF-α）分泌量。此外，使用TLR4特异性抑制剂可显著介导κ-卡拉胶多糖诱导的TNF-α分泌增加。用κ-卡拉胶多糖处理的RAW 264.7细胞显示TLR4表达上调，且NF-κB的主要亚基（p65）发生易位。这些结果支持κ-卡拉胶多糖在RAW 264.7细胞中的免疫调节功能。
