Blackwood Sarah J, Dwyer Renee M, Bradley Eloise A, Keske Michelle A, Richards Stephen M, Rattigan Stephen
Menzies Institute for Medical Research, University of Tasmania, Hobart, Australia.
School of Medicine, University of Tasmania, Hobart, Australia.
Ultrasound Med Biol. 2017 Sep;43(9):2013-2023. doi: 10.1016/j.ultrasmedbio.2017.05.012. Epub 2017 Jun 24.
Most methods of assessing flowmotion (rhythmic oscillation of blood flow through tissue) are limited to small sections of tissue and are invasive in tissues other than skin. To overcome these limitations, we adapted the contrast-enhanced ultrasound (CEUS) technique to assess microvascular flowmotion throughout a large region of tissue, in a non-invasive manner and in real time. Skeletal muscle flowmotion was assessed in anaesthetised Sprague Dawley rats, using CEUS and laser Doppler flowmetry (LDF) for comparison. Wavelet transformation of CEUS and LDF data was used to quantify flowmotion. The α-adrenoceptor antagonist phentolamine was infused to predictably blunt the neurogenic component of flowmotion. Both techniques identified similar flowmotion patterns, validating the use of CEUS to assess flowmotion. This study demonstrates for the first time that the novel technique of CEUS can be adapted for determination of skeletal muscle flowmotion in large regions of skeletal muscle.
大多数评估血流运动（血液流经组织的节律性振荡）的方法仅限于小部分组织，并且对皮肤以外的组织具有侵入性。为了克服这些限制，我们采用了对比增强超声（CEUS）技术，以非侵入性且实时的方式评估整个大区域组织中的微血管血流运动。在麻醉的Sprague Dawley大鼠中评估骨骼肌血流运动，并使用CEUS和激光多普勒血流仪（LDF）进行比较。对CEUS和LDF数据进行小波变换以量化血流运动。注入α-肾上腺素能受体拮抗剂酚妥拉明，以可预测的方式减弱血流运动的神经源性成分。两种技术都识别出相似的血流运动模式，验证了使用CEUS评估血流运动的有效性。这项研究首次证明，CEUS新技术可用于确定骨骼肌大区域中的骨骼肌血流运动。
