Gustafsson U, Gidlöf A, Lewis D H, Sollevi A
Clinical Research Center, Faculty of Health Sciences, University Hospital, Linköping, Sweden.
Int J Microcirc Clin Exp. 1994 Sep-Oct;14(5):303-7. doi: 10.1159/000178846.
The aim of this study was to investigate the characteristics of the microcirculatory blood flow with laser-Doppler flowmetry (LDF) in skeletal muscle during both regional and systemic infusions of adenosine. A laser-Doppler flowmeter probe was placed on the left gastrocnemius muscle in anesthetized rats. Adenosine was given either systemically in the jugular vein (group A) or administered regionally in the iliac artery (group B). The infusion of adenosine was increased stepwise by 50 micrograms/kg/min, every 10 min, up to 400 micrograms/kg/min. In group A there was a dose-dependent decrease in mean arterial pressure as well as in LDF flow; flowmotion of the LDF signal with a frequency of 1.6 cycles/min was seen at a mean adenosine dose of 240 micrograms/kg/min at a blood pressure of 60 mm Hg. In group B there was a dose-dependent decrease of mean arterial blood pressure but not as marked compared to group A, meanwhile the LDF flow was unchanged; flowmotion was seen at a mean adenosine dose of 220 microgram/kg/min at a blood pressure of 72 mm Hg, with a frequency of 1.5 cycles/min. This study demonstrates that adenosine, given either regionally intraarterially with maintained blood flow, or intravenously with reduced blood flow, induces flowmotion in skeletal muscle microcirculation with a frequency of approximately 1.5 cycles/min.
本研究的目的是利用激光多普勒血流仪（LDF）研究在局部和全身输注腺苷期间骨骼肌微循环血流的特征。将激光多普勒血流仪探头置于麻醉大鼠的左腓肠肌上。腺苷通过颈静脉进行全身给药（A组）或通过髂动脉进行局部给药（B组）。每10分钟将腺苷输注量以50微克/千克/分钟的幅度逐步增加，直至400微克/千克/分钟。在A组中，平均动脉压以及LDF血流呈剂量依赖性降低；在平均腺苷剂量为240微克/千克/分钟、血压为60毫米汞柱时，观察到LDF信号以1.6次/分钟的频率出现血流波动。在B组中，平均动脉血压呈剂量依赖性降低，但与A组相比不那么明显，同时LDF血流未发生变化；在平均腺苷剂量为220微克/千克/分钟、血压为72毫米汞柱时，观察到血流波动，频率为1.5次/分钟。本研究表明，无论是在维持血流的情况下进行动脉内局部给药，还是在血流减少的情况下进行静脉给药，腺苷均可诱导骨骼肌微循环出现频率约为1.5次/分钟的血流波动。
