超高效液相色谱-串联质谱法同时测定血浆中氟西汀、利培酮及其活性代谢物9-羟基利培酮：在大鼠药代动力学研究中的应用

UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats.

作者信息

Ezzeldin Essam, Abo-Talib Nisreen F, Tammam Marwa H

机构信息

Pharmaceutical Chemistry Department and Drug Bioavailability Laboratory, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.

Drug Bioavailability Center, National Organization for Drug Control and Research, P.O. Box 29, Cairo, Egypt.

出版信息

J Anal Methods Chem. 2017;2017:5187084. doi: 10.1155/2017/5187084. Epub 2017 Jun 1.

Risperidone (RIS) is used as an antipsychotic drug alone or with other drugs, like fluoxetine (FLX). A simple method was developed and validated for the determination of both RIS and its metabolite 9-hydroxyrisperidone (9-OH-RIS), FLX, and olanzapine (OLA) as an internal standard in rat's plasma using UPLC-MS/MS. FLX, RIS, 9-OH-RIS, and OLA were purified using acetonitrile as a protein precipitating agent. Separation was performed on an ACQUITY™ "UPLC BEH™" C18 column (50 mm × 2.1 mm i.d., 1.7 m; Waters Corp., USA). The ranges of the calibration curves were 1.0-1000.0 ng/mL for FLX and 0.2-1000.0 ng/mL for RIS and 9-OH-RIS. Linearity, recovery, precision, and stability were within the acceptable range. This method is rapid, fast, and precise for the determination of RIS and FLX in plasma and is applicable in pharmacokinetic studies.

利培酮（RIS）单独或与其他药物（如氟西汀（FLX））一起用作抗精神病药物。开发并验证了一种简单的方法，用于使用超高效液相色谱-串联质谱法（UPLC-MS/MS）测定大鼠血浆中RIS及其代谢物9-羟基利培酮（9-OH-RIS）、FLX和作为内标的奥氮平（OLA）。使用乙腈作为蛋白质沉淀剂对FLX、RIS、9-OH-RIS和OLA进行纯化。在ACQUITY™ “UPLC BEH™” C18柱（50 mm×2.1 mm内径，1.7 µm；美国沃特世公司）上进行分离。FLX的校准曲线范围为1.0 - 1000.0 ng/mL，RIS和9-OH-RIS的校准曲线范围为0.2 - 1000.0 ng/mL。线性、回收率、精密度和稳定性均在可接受范围内。该方法快速、准确，可用于测定血浆中的RIS和FLX，适用于药代动力学研究。