Department of Orthopedics, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, P.R. China.
Mol Med Rep. 2017 Aug;16(2):1661-1668. doi: 10.3892/mmr.2017.6811. Epub 2017 Jun 20.
Spinal cord injury is a serious threat to human health and various techniques have been deployed to ameliorate or cure its effects. Stem cells transplantation is one of the promising methods. The primary aim of the present study was to investigate the effect of the transplantation of olfactory ensheathing cell (OEC) conditioned medium‑induced bone marrow stromal cells (BMSCs) on spinal cord injury. Rat spinal cord compression injury animal models were generated, and the rats divided into the following three groups: Group A, (control) Dulbecco's modified Eagle's medium‑treated group; group B, normal BMSC‑treated group; group C, OEC conditioned medium‑induced BMSC‑treated group. The animals were sacrificed at 2, 4 and 8 weeks following transplantation for hematoxylin and eosin staining, and fluorescence staining of neurofilament protein, growth associated protein‑43 and neuron‑specific nuclear protein. The cavity area of the spinal cord injury was significantly reduced at 2 and 4 weeks following transplantation in group C, and a significant difference between the Basso, Beattie and Bresnahan score in group C and groups A and B was observed. Regenerated nerve fibers were observed in groups B and C; however, a greater number of regenerated nerve fibers were observed in group C. BMSCs induced by OEC conditioned medium survived in vivo, significantly reduced the cavity area of spinal cord injury, promoted nerve fiber regeneration following spinal cord injury and facilitated recovery of motor function. The present study demonstrated a novel method to repair spinal cord injury by using induced BMSCs, with satisfactory results.
脊髓损伤是对人类健康的严重威胁，已经采用了各种技术来改善或治愈其影响。干细胞移植是一种很有前途的方法。本研究的主要目的是研究嗅鞘细胞（OEC）条件培养基诱导的骨髓基质细胞（BMSC）移植对脊髓损伤的影响。建立大鼠脊髓压迫损伤动物模型，并将大鼠分为以下三组：A 组（对照组），给予 DMEM 处理；B 组，给予正常 BMSC 处理；C 组，给予 OEC 条件培养基诱导的 BMSC 处理。移植后 2、4 和 8 周处死动物，行苏木精-伊红染色，神经丝蛋白、生长相关蛋白-43 和神经元特异性核蛋白荧光染色。C 组在移植后 2 和 4 周时脊髓损伤的腔面积明显缩小，C 组与 A 组和 B 组之间的 Basso、Beattie 和 Bresnahan 评分有显著差异。B 组和 C 组均观察到再生神经纤维，但 C 组的再生神经纤维较多。OEC 条件培养基诱导的 BMSC 在体内存活，显著减少脊髓损伤的腔面积，促进脊髓损伤后神经纤维再生，并促进运动功能恢复。本研究通过使用诱导的 BMSC 为修复脊髓损伤提供了一种新方法，取得了满意的效果。
