利用负压和正压通过血管和气道对完整肺组织进行去细胞化处理。
Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.
作者信息
Skolasinski Steven, Panoskaltsis-Mortari Angela
机构信息
Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and sleep, 350 Variety Heart and Research Center, University of Minnesota, 420 Delaware St. SE, MMC 276, Minneapolis, MN, 55455, USA.
Department of Pediatrics, Blood and Marrow Transplant Program, University of Minnesota, Mayo Mail Code 366, 420 Delaware Street SE, Minneapolis, MN, 55455, USA.
出版信息
Methods Mol Biol. 2018;1577:307-315. doi: 10.1007/7651_2017_32.
Abstract
Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.
摘要
去细胞化可产生细胞外基质（ECM）支架。在此，我们描述了利用正压和负压相结合的方法，分别将去细胞化试剂驱入结构完整的肺的脉管系统和气道，以去除细胞和细胞物质，从而留下完整的ECM支架。
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