比较卵胞浆内单精子注射（ICSI）和体外受精（IVF）病例中的非整倍体率。

To compare aneuploidy rates between ICSI and IVF Cases.

作者信息

Sahin L, Bozkurt M, Şahin H, Gürel A, Calıskan E

机构信息

Department in Obstetrics and Gynecology, Kafkas University School of Medicine, Kars, Turkey.

Department in Obstetrics and Gynecology, Sakarya University School of Medicine, Sakarya, Turkey.

出版信息

Niger J Clin Pract. 2017 Jun;20(6):652-658. doi: 10.4103/1119-3077.208959.

DOI:10.4103/1119-3077.208959

PMID:28656917

Abstract

INTRODUCTION

Intracytoplasmic sperm injection (ICSI) currently helps many couples with male infertility. However, ICSI procedure may cause asynchronous sperm decondensation. This could introduce a risk for aneuploidy. The ICSI technique also could cause damage to the second meiotic spindle during injection and cause significantly abnormal pairing of chromosomes when compared with In vitro fertilization (IVF). In this study, we have examined whether ICSI has a higher incidence of aneuploidy when compared with IVF.

MATERIAL AND METHODS

A retrospective study was conducted on 36 individuals. Common numbers of chromosome abnormalities were detected using fluorescent in-situ hybridization (FISH). Seven probes were used to detect chromosome X, Y, 13, 16, 18, 21, and 22. Chi-square test was used for statistical analysis and presented as odd ratios with confidence intervals.

RESULTS

The age range was 26 through 44 (mean age 35.5) for IVF and 25 through 46 (mean age 35.8) for ICSI. From the 36 egg retrievals, 57 embryos were obtained from nine individuals using IVF and 183 embryos were obtained from 27 individuals using ICSI. For the IVF group, 37 of the 57 examined embryos were abnormal (65%), whereas 128 of 183 examined embryos were abnormal for the ICSI group (69.9%). Among the 57 embryos from the IVF cases, the number of absolute abnormal chromosomes were as follows: X&Y chromosomes: 4 (12.9%), chromosome 13: 9 (29%), chromosome 16: 7 (22.5%), chromosome 18: 6 (19.3%), chromosome 21: 8 (25.8%), chromosome 22: 10 (32.2%). For the ICSI embryos: X and Y chromosomes: 18 (14%), chromosome 13: 34 (26.5%), chromosome 16: 23 (18%), chromosome 18: 23 (18%), chromosome 21: 26 (20.3%), chromosome 22: 31 (24.2%). The odds ratios for the difference between IVF and ICSI for each chromosome were as follows: X&Y chromosomes: 1.53 (0.598-3.916), chromosome 13: 0.969 (0.443-2.122), chromosome 16: 0.709 (0.307-1.639), chromosome 18: 1.650 (0.650-4.188), chromosome 21: 0.777 (0.350-1.724), chromosome 22: 0.647 (0.311-1.348). Overall no significant difference between two insemination procedures was seen 0.948 (0.678-1.324).

CONCLUSIONS

As a result; ICSI does not create a significantly higher aneuploidy number when compared with IVF as examined by FISH analysis of seven chromosome pairs.

摘要

引言

胞浆内单精子注射（ICSI）目前帮助了许多患有男性不育症的夫妇。然而，ICSI操作可能会导致精子去浓缩不同步。这可能会带来非整倍体的风险。与体外受精（IVF）相比，ICSI技术在注射过程中也可能会对第二次减数分裂纺锤体造成损伤，并导致染色体配对明显异常。在本研究中，我们研究了与IVF相比，ICSI导致非整倍体的发生率是否更高。

材料与方法

对36名个体进行了回顾性研究。使用荧光原位杂交（FISH）检测常见的染色体异常数量。使用7种探针检测染色体X、Y、13、16、18、21和22。采用卡方检验进行统计分析，并以带有置信区间的比值比表示。

结果

IVF组的年龄范围为26至44岁（平均年龄35.5岁），ICSI组为25至46岁（平均年龄35.8岁）。从36次取卵中，9名个体通过IVF获得了57个胚胎，27名个体通过ICSI获得了183个胚胎。对于IVF组，57个检测胚胎中有37个异常（65%），而ICSI组183个检测胚胎中有128个异常（69.9%）。在IVF病例的57个胚胎中，绝对异常染色体的数量如下：X和Y染色体：4个（12.9%），13号染色体：9个（29%），16号染色体：7个（22.5%），18号染色体：6个（19.3%），21号染色体：8个（25.8%），22号染色体：10个（32.2%）。对于ICSI胚胎：X和Y染色体：18个（14%），13号染色体：34个（26.5%），16号染色体：23个（18%），18号染色体：23个（18%），21号染色体：26个（20.3%），22号染色体：31个（24.2%）。IVF和ICSI之间每条染色体差异的比值比分别如下：X和Y染色体：1.53（0.598 - 3.916），13号染色体：0.969（0.443 - 2.122），16号染色体：0.709（0.307 - 1.639），18号染色体：1.650（0.650 - 4.188），21号染色体：0.777（0.350 - 1.724），22号染色体：0.647（0.311 - 1.348）。总体而言，两种授精程序之间未观察到显著差异，比值比为0.948（0.678 - 1.324）。