Bekhterev V N, Gavrilova S N, Koshkareva E V, Shipanov I N
Sochi State University, Sochi, Russia, 354000; Krasnodar Bureau of Forensic Medical Expertise #2, Ministry of Health of the Krasnodar Region, Sochi, Russia, 354000.
Krasnodar Bureau of Forensic Medical Expertise #2, Ministry of Health of the Krasnodar Region, Sochi, Russia, 354000.
Sud Med Ekspert. 2017;60(3):27-31. doi: 10.17116/sudmed201760327-31.
The express-method for the determination of pyrovaleron in the urine based on the combination with the method of extractive freezing-out and centrifugation of the samples as the preliminary stage of the preparation of a biological object for the analysis. The identification and quantitative determination of the substance of interest were performed using gas chromatography with nitrogen-selective detection. The preparation of the samples was carried out as a single-step procedure no longer than 30 min in duration. The limit of alpha-pyrovaleron detection in the urine was estimated at 1 mcg/ml. Its concentration after extraction from the urine increased by a factor of more than nine.
基于将样品的萃取冷冻分离和离心方法相结合作为生物分析对象制备的初步阶段，尿液中吡咯戊酮的快速测定方法。使用带氮选择性检测的气相色谱法对目标物质进行鉴定和定量测定。样品制备作为一个持续时间不超过30分钟的单步程序进行。尿液中α-吡咯戊酮的检测限估计为1微克/毫升。从尿液中萃取后其浓度提高了九倍多。
