Christensson-Nylander I, Terenius L
Neuropeptides. 1985 Sep;6(5):391-6. doi: 10.1016/0143-4179(85)90137-4.
Stepwise processing of the prohormone for dynorphin-related peptides, proenkephalin B, may generate a large number of opioid peptides. It is therefore important to perform a chemical characterization of the immunoreactive dynorphin (ir-dyn) found in different tissues. In this study dynorphin peptides in human substantia nigra were characterized. Highly sensitive radioimmunoassays (RIA) directed against the C-terminals of dynorphin A (dyn A) and dynorphin B (dyn B) respectively, were used to measure the levels of dynorphin peptides. Tissue extraction was performed either with MeOH/0.1 M HCl (1/1) or 1 M HAc. Gel filtration on a Sephadex G-50 column revealed three peaks of ir-dyn B, the predominating one coeluting with synthetic dyn B. Ir-dyn A also appeared in three peaks, one of them (20-30%) coeluting with dyn A. The HAc extract contained much higher levels of ir-dyn B as compared to the MeOH/HCl extract, but approximately the same levels of ir-dyn A. Ion exchange separation of the HAc extract somewhat changed the size distribution pattern, with more ir coeluting with the synthetic peptides compared to other molecular weight forms.
强啡肽相关肽前激素原脑啡肽B的逐步加工过程可能会产生大量阿片肽。因此，对不同组织中发现的免疫反应性强啡肽（ir-dyn）进行化学表征非常重要。在本研究中，对人黑质中的强啡肽肽进行了表征。分别针对强啡肽A（dyn A）和强啡肽B（dyn B）的C末端的高灵敏度放射免疫分析（RIA）用于测量强啡肽肽的水平。组织提取采用甲醇/0.1 M盐酸（1/1）或1 M醋酸进行。在Sephadex G-50柱上进行凝胶过滤，显示出三个ir-dyn B峰，其中占主导地位的一个与合成dyn B共洗脱。Ir-dyn A也出现在三个峰中，其中一个（20-30%）与dyn A共洗脱。与甲醇/盐酸提取物相比，醋酸提取物中ir-dyn B的水平要高得多，但ir-dyn A的水平大致相同。醋酸提取物的离子交换分离在一定程度上改变了大小分布模式，与其他分子量形式相比，与合成肽共洗脱的ir更多。
