Ham Jiyeon, Lim Whasun, Bazer Fuller W, Song Gwonhwa
Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Department of Biomedical Sciences, Catholic Kwandong University, Gangneung, Republic of Korea.
J Cell Physiol. 2018 Feb;233(2):1638-1649. doi: 10.1002/jcp.26069. Epub 2017 Jul 24.
Silibinin is a flavonolignan extracted from seeds of milk thistles. Traditionally, it has been used as a therapeutic agent for liver disorders, and now it is well-known for its anti-cancer effects. However, studies on anti-cancer effects of silibinin on choriocarcinoma are very limited. Therefore, we performed proliferation and apoptosis assays to determine effects of silibinin on the viability of human choriocarcinoma (JAR and JEG3) cells. Our results showed that silibinin significantly inhibited proliferation and induced apoptosis in both JAR and JEG3 cells, and significantly increased reactive oxygen species (ROS) and lipid peroxidation. Moreover, silibinin disrupted mitochondrial function by inducing permeabilization of mitochondrial membrane potential and calcium ion efflux in JAR and JEG3 cells. Furthermore, silibinin-induced apoptosis in choriocarcinoma cells via AKT, mitogen-activated protein kinases (MAPK) and unfolded protein response (UPR) signal transduction. Collectively, our results suggest that silibinin is a novel therapeutic agent or dietary supplement for management of human placental choriocarcinomas.
水飞蓟宾是从水飞蓟种子中提取的一种黄酮木脂素。传统上，它一直被用作治疗肝脏疾病的药物，现在它因其抗癌作用而闻名。然而，关于水飞蓟宾对绒毛膜癌抗癌作用的研究非常有限。因此，我们进行了增殖和凋亡检测，以确定水飞蓟宾对人绒毛膜癌（JAR和JEG3）细胞活力的影响。我们的结果表明，水飞蓟宾显著抑制JAR和JEG3细胞的增殖并诱导其凋亡，且显著增加活性氧（ROS）和脂质过氧化。此外，水飞蓟宾通过诱导JAR和JEG3细胞线粒体膜电位通透性和钙离子外流来破坏线粒体功能。此外，水飞蓟宾通过AKT、丝裂原活化蛋白激酶（MAPK）和未折叠蛋白反应（UPR）信号转导诱导绒毛膜癌细胞凋亡。总的来说，我们的结果表明水飞蓟宾是一种用于治疗人胎盘绒毛膜癌的新型治疗剂或膳食补充剂。
