水飞蓟素纳米载药对 HT-29 人结肠癌细胞的影响。

Effects of Silymarin-Loaded Nanoparticles on HT-29 Human Colon Cancer Cells.

机构信息

Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran.

Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran.

出版信息

Medicina (Kaunas). 2018 Mar 9;54(1):1. doi: 10.3390/medicina54010001.

: Previous studies have demonstrated the anti-cancer effects of silymarin (SLM). However, the low bioavailability of SLM has restricted its use. This study investigated the toxic effect of nanostructured SLM encapsulated in micelles (Nano-SLM) on the growth of the HT-29 human colon cancer cell line. : HT-29 cells were treated with 25 μM/mL of SLM or Nano-SLM for 48 h. MTT and colony formation assays were used to assess the cytotoxicity and proliferation of HT-29 cells, respectively. The cells were stained with annexin V/PI for assessment of apoptosis. : MTT assays revealed that Nano-SLM treatment was able to exert a more pronounced toxic effect on the HT-29 cells as compared to free SLM treatment ( < 0.01). In the Nano-SLM-treated cells, colony numbers were significantly reduced in comparison to the free SLM-treated cells ( < 0.01). Apoptotic and necrotic indexes of Nano-SLM-treated HT-29 cells were also significantly increased in comparison to those of the free SLM-treated cells ( < 0.01). The viability, proliferation and apoptosis of healthy cells (NIH-3T3 cells) were not changed in response to Nano-SLM or SLM. : Our results indicate that Nano-SLM enhances the anti-cancer effects of SLM against human colon cancer cells.

: 先前的研究已经证明了水飞蓟素（SLM）的抗癌作用。然而，SLM 的生物利用度低限制了其应用。本研究探讨了纳米结构的 SLM 封装在胶束中的毒性作用（Nano-SLM）对 HT-29 人结肠癌细胞系生长的影响。 : 将 HT-29 细胞用 25 μM/mL 的 SLM 或 Nano-SLM 处理 48 小时。MTT 和集落形成试验分别用于评估 HT-29 细胞的细胞毒性和增殖。用 Annexin V/PI 染色评估细胞凋亡。 : MTT 试验表明，与游离 SLM 处理相比，Nano-SLM 处理能更显著地对 HT-29 细胞产生毒性作用（<0.01）。在 Nano-SLM 处理的细胞中，与游离 SLM 处理的细胞相比，集落数量明显减少（<0.01）。与游离 SLM 处理的细胞相比，Nano-SLM 处理的 HT-29 细胞的凋亡和坏死指数也显著增加（<0.01）。Nano-SLM 或 SLM 对健康细胞（NIH-3T3 细胞）的活力、增殖和凋亡没有影响。 : 我们的结果表明，Nano-SLM 增强了 SLM 对人结肠癌细胞的抗癌作用。