Johnson T R, Ilan J
Proc Natl Acad Sci U S A. 1985 Nov;82(21):7327-9. doi: 10.1073/pnas.82.21.7327.
The messenger RNA for chicken serum albumin together with its specific binding proteins was purified by hybrid selection using filter-bound cloned albumin cDNA. Under conditions where hybridization of the protein-mRNA complex was specific for the immobilized cDNA sequence, no dissociation of the complex into its protein and RNA components was apparent. Electrophoretic analysis of albumin mRNA-specific binding proteins showed three major bands. Two of these were prominent in total poly(A) messenger ribonucleoprotein. The third band was of much greater relative intensity in the albumin mRNA-specific proteins than in total poly(A) messenger ribonucleoprotein. The results suggest that the proteins bound to albumin mRNA represent only a subset of the total population of poly(A) mRNA-associated protein.
利用固定在滤膜上的克隆白蛋白互补脱氧核糖核酸（cDNA），通过杂交筛选法纯化了鸡血清白蛋白信使核糖核酸（mRNA）及其特异性结合蛋白。在蛋白质 - mRNA复合物的杂交对固定的cDNA序列具有特异性的条件下，复合物未明显解离成其蛋白质和RNA组分。对白蛋白mRNA特异性结合蛋白的电泳分析显示出三条主要条带。其中两条在总的聚腺苷酸（poly(A)）信使核糖核蛋白中很突出。第三条带在白蛋白mRNA特异性蛋白中的相对强度比在总的poly(A)信使核糖核蛋白中要大得多。结果表明，与白蛋白mRNA结合的蛋白质仅代表与poly(A) mRNA相关蛋白总群体的一个子集。
