França Monica Malheiros, Lerario Antonio M, Fragoso Maria Candida B V, Lotfi Claudimara Ferini Pacicco
Departamento de Anatomia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Laboratorio de Hormonio e Genetica Molecular (LIM-42), Unidade Adrenal, Divisao de Endocrinologia, Faculdade de Medicine, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Clinics (Sao Paulo). 2017 Jun;72(6):391-394. doi: 10.6061/clinics/2017(06)10.
: Transcription Factor 21 represses steroidogenic factor 1, a nuclear receptor required for gonadal development, sex determination and the regulation of adrenogonadal steroidogenesis. The aim of this study was to investigate whether silencing or overexpression of the gene Transcription Factor 21 could modulate the gene and protein expression of steroidogenic factor 1 in adrenocortical tumors.
: We analyzed the gene expression of steroidogenic factor 1 using qPCR after silencing endogenous Transcription Factor 21 in pediatric adrenal adenoma-T7 cells through small interfering RNA. In addition, using overexpression of Transcription Factor 21 in human adrenocortical carcinoma cells, we analyzed the protein expression of steroidogenic factor 1 using Western blotting.
: Transcription Factor 21 knockdown increased the mRNA expression of steroidogenic factor 1 by 5.97-fold in pediatric adrenal adenoma-T7 cells. Additionally, Transcription Factor 21 overexpression inhibited the protein expression of steroidogenic factor 1 by 0.41-fold and 0.64-fold in two different adult adrenocortical carcinoma cell cultures, H295R and T36, respectively.
: Transcription Factor 21 is downregulated in adrenocortical carcinoma cells. Taken together, these findings support the hypothesis that Transcription Factor 21 is a regulator of steroidogenic factor 1 and is a tumor suppressor gene in pediatric and adult adrenocortical tumors.
转录因子21可抑制类固醇生成因子1，后者是性腺发育、性别决定及肾上腺性腺类固醇生成调节所需的一种核受体。本研究旨在探究转录因子21基因的沉默或过表达是否能调节肾上腺皮质肿瘤中类固醇生成因子1的基因和蛋白表达。
我们通过小干扰RNA沉默小儿肾上腺腺瘤-T7细胞中的内源性转录因子21后，使用定量聚合酶链反应分析类固醇生成因子1的基因表达。此外，通过在人肾上腺皮质癌细胞中过表达转录因子21，我们使用蛋白质免疫印迹法分析类固醇生成因子1的蛋白表达。
在小儿肾上腺腺瘤-T7细胞中，转录因子21敲低使类固醇生成因子1的mRNA表达增加了5.97倍。此外，在两种不同的成人肾上腺皮质癌细胞株H295R和T36中，转录因子21过表达分别使类固醇生成因子1的蛋白表达抑制了0.41倍和0.64倍。
肾上腺皮质癌细胞中转录因子21表达下调。综上所述，这些发现支持以下假说：转录因子21是类固醇生成因子1的调节因子，并且是小儿和成人肾上腺皮质肿瘤中的一种肿瘤抑制基因。
