Sylvester Marisa L, Ratner Buddy D
Department of Bioengineering, University of Washington, Seattle, Washington 98195.
Biointerphases. 2017 Jun 28;12(2):02C419. doi: 10.1116/1.4989835.
The development of biomaterials that promote tissue reconstruction and regeneration can reduce the low level, chronic inflammation and encapsulation that impact the performance of today's medical devices. Specifically, in the case of implantable sensors, the host response often leads to poor device performance that discourages permanent implantation. Our goal is to present on medical implants bioactive molecules that can promote healing rather than scarring. Localized delivery of these molecules would also minimize the possibility of adverse tissue reactions elsewhere in the body. Toward this end, the authors have developed a collagen affinity coating that binds a number of potential healing molecules and can be attached to the surface of an implanted biomaterial. This allows the creation of a wide variety of natural surface coatings that can be evaluated and tailored to promote the desired healing response. To demonstrate the efficacy of this collagen affinity coating to biospecifically bind promising healing molecules to type I collagen in vivo, the antifibrotic proteoglycan decorin was utilized. Decorin binds and renders ineffective the protein transforming growth factor beta (TGFβ) that induces collagen scar production. Thus, an assembled, supramolecular structure of biomaterial-collagen-decorin-TGFβ is formed. A decorin surface coating was created and characterized, illustrating the potential of this type I collagen affinity coating for widespread use with a variety of promising healing molecules. Future studies will test the implant efficacy of this type of coating.
促进组织重建和再生的生物材料的开发可以减少影响当今医疗设备性能的低度慢性炎症和包膜形成。具体而言，对于可植入传感器，宿主反应常常导致设备性能不佳，从而阻碍永久植入。我们的目标是在医疗植入物上呈现能够促进愈合而非瘢痕形成的生物活性分子。这些分子的局部递送还将使身体其他部位出现不良组织反应的可能性降至最低。为此，作者开发了一种胶原亲和涂层，该涂层可结合多种潜在的愈合分子，并能附着于植入生物材料的表面。这使得能够创建各种各样的天然表面涂层，可对其进行评估和定制，以促进所需的愈合反应。为了证明这种胶原亲和涂层在体内将有前景的愈合分子生物特异性结合到I型胶原上的功效，使用了抗纤维化蛋白聚糖核心蛋白聚糖。核心蛋白聚糖能结合并使诱导胶原瘢痕产生的蛋白质转化生长因子β（TGFβ）失效。因此，形成了生物材料 - 胶原 - 核心蛋白聚糖 - TGFβ的组装超分子结构。创建并表征了核心蛋白聚糖表面涂层，说明了这种I型胶原亲和涂层与多种有前景的愈合分子广泛联用的潜力。未来的研究将测试这种涂层的植入效果。
