Karabulut Bilge, Karaman Nihan Ayyildiz, Caydere Muzaffer, Karabulut Ramazan
Department of Pediatric Surgery, Ankara Training and Research Hospital, Gezegen Sokak 1/10, Gaziosmanpasa 06670, Cankaya, Ankara, Turkey.
Department of Pathology, Ankara Training and Research Hospital, Ankara, Turkey.
Indian J Surg. 2017 Jun;79(3):201-205. doi: 10.1007/s12262-016-1465-2. Epub 2016 Mar 17.
The aim of this study was to determine the effect of splenectomy in the short bowel syndrome. Twenty-four Wistar-albino rats weighing between 210 and 375 g were used. They were divided into three groups. In group A, short bowel syndrome (SBS) was created by 75 % bowel resection. In group B, SBS and splenectomy was performed. In group C, after transecting the bowel, it was anastomosed. Before and 45 days after the procedures, all rats were weighed. In all three groups, the first and final weight of the rats, the final bowel weight and length, the ileal and jejunal crypt depths, the villus height, the luminal diameter, the bowel wall thickness, and the number of apoptotic cells and mitosis per 100 crypt cell were compared. Periportal fibrosis, infiltration, bile stasis, and bile duct proliferation were detected in liver samples. The rat intestinal length and weight was the least in group B while the jejunal crypt depth was higher in group B than in group A and it was exactly the opposite for the jejunal and ileal villus heights. The ileal and jejunal luminal diameter, the ileal bowel wall thickness, the jejunal and ileal apoptotic cell number, the jejunal mitosis, and the periportal fibrosis were highest in group B. Adding splenectomy to an SBS model has a negative impact on bowel adaptation.
本研究的目的是确定脾切除术对短肠综合征的影响。使用了24只体重在210至375克之间的Wistar白化大鼠。它们被分为三组。A组通过75%肠切除术造成短肠综合征（SBS）。B组进行了SBS和脾切除术。C组在切断肠管后进行吻合。在手术前和手术后45天，对所有大鼠进行称重。比较了三组大鼠的初始体重和最终体重、最终肠重量和长度、回肠和空肠隐窝深度、绒毛高度、管腔直径、肠壁厚度以及每100个隐窝细胞中的凋亡细胞数和有丝分裂数。在肝脏样本中检测到门周纤维化、浸润、胆汁淤积和胆管增生。B组大鼠的肠长度和重量最小，而B组空肠隐窝深度高于A组，空肠和回肠绒毛高度则相反。B组的回肠和空肠管腔直径、回肠肠壁厚度、空肠和回肠凋亡细胞数、空肠有丝分裂以及门周纤维化最高。在SBS模型中增加脾切除术对肠适应有负面影响。
