Komur Baran, Akyuva Yener, Karaslan Numan, Isyar Mehmet, Gumustas Seyit Ali, Yilmaz Ibrahim, Akkaya Semih, Sirin Duygu Yasar, Mutlu Cagri Ata, Batmaz Ahmet Guray, Guler Olcay, Mahirogullari Mahir
Kanuni Sultan Süleyman Training and Research Hospital, Department of Orthopaedics and Traumatology, 34303 Istanbul, Turkey.
Ministry of Health, Department of Neurosurgery, 59100 Tekirdag, Turkey.
J Pharm (Cairo). 2017;2017:7457865. doi: 10.1155/2017/7457865. Epub 2017 Jun 4.
Use of biodegradable and biocompatible materials in the orthopedic surgery is gaining popularity. In this research, the rate of controlled release of a bilayered prototype biomaterial designed to promote osteoblastic and tenoblastic activity was calculated using pharmacochemical methods.
The first part of the design, composed of a sodium tetraborate, polyvinyl alcohol, and starch based hydrogel, was loaded with bone morphogenic protein-2. The second part which was composed of a sodium tetraborate, polyvinyl alcohol, and chitosan based hydrogel was loaded with bone morphogenic protein-12. Osteochondral and tendon tissue specimens were obtained from patients with a diagnosis of gonarthrosis and primary bone cells and tendon cells cultures were prepared following treatment with collagenase enzyme. Cell samples were collected from the groups by means of an invert light microscope and environmental scanning electron microscope underwent at the 1st and 21st days. The level of osteogenic differentiation was measured by the activity of alkaline phosphatase. For the statistical evaluation of the obtained data, groups were compared with post hoc Tukey test following analysis of variance. Level of significance was accepted to be <0,01.
Both osteogenic and tenogenic stimulation were observed in the cultured specimens. In comparison to the control groups, the rate of proliferation of healthy cells was found to be higher in the groups to which the design was added ( < 0.01).
Our research is a preliminary report that describes a study conducted in an in vitro experimental setting. We believe that such prototype systems may be pioneers in targeted drug therapies after reconstructional surgeries.
可生物降解和生物相容性材料在骨科手术中的应用越来越普遍。在本研究中，使用药物化学方法计算了一种旨在促进成骨细胞和肌腱细胞活性的双层原型生物材料的控释速率。
设计的第一部分由硼酸钠、聚乙烯醇和淀粉基水凝胶组成，负载骨形态发生蛋白-2。第二部分由硼酸钠、聚乙烯醇和壳聚糖基水凝胶组成，负载骨形态发生蛋白-12。从诊断为膝关节炎的患者身上获取骨软骨和肌腱组织标本，并在用胶原酶处理后制备原代骨细胞和肌腱细胞培养物。在第1天和第21天通过倒置光学显微镜收集各实验组的细胞样本，并进行环境扫描电子显微镜观察。通过碱性磷酸酶活性测量成骨分化水平。对于所获得数据的统计评估，在方差分析后，采用事后Tukey检验对各实验组进行比较。显著性水平设定为<0.01。
在培养的标本中观察到了成骨和肌腱生成刺激。与对照组相比，添加该设计的实验组中健康细胞的增殖率更高（<0.01）。
我们的研究是一份初步报告，描述了在体外实验环境中进行的一项研究。我们相信，这种原型系统可能成为重建手术后靶向药物治疗的先驱。
