van Marle J, Piek T, Lammertse T, Lind A, Van Weeren-Kramer J
Brain Res. 1985 Nov 25;348(1):107-11. doi: 10.1016/0006-8993(85)90365-8.
The common inhibitor (CI) and slow excitor tibiae (SETi) innervated slow muscles 135cd of the locust Schistocerca gregaria were incubated under high-affinity uptake conditions either in [3H]GABA or in [3H]glutamate. [3H]GABA is accumulated in the glia of the nerve endings of the CI as well as the SETi; however, it is accumulated only in the terminal axons of the CI, not in the terminal axons of the SETi. The grain densities above the glia and above the CI terminal axons are approximately 2 grains/micron2. After incubation in [3H]glutamate the grain densities above the CI terminal axons and the SETi terminal axons are approximately 4 grains/micron2; the grain densities above the glia of both types of nerve endings are approximately 17 grains/micron2. The relatively high labeling (3 grains/micron2) of the muscles after incubation in the presence of glutamate is ascribed to the high metabolic requirements of slow muscles. The conclusion is drawn that a high-affinity uptake system for GABA is present in the CI terminal axons and in the glia of both the CI and SETi nerve endings. However, while the glutamate uptake in the CI and SETi nerve endings of the slow 135cd is comparable to the high-affinity uptake of glutamate in the fast excitor tibiae (FETi) nerve endings of the fast retractor unguis muscle, a high-affinity uptake of glutamate was only demonstrated in the glia of both types of nerve endings. A high-affinity uptake in the terminal axons of the CI and SETi may be masked by an extensively low-affinity uptake of glutamate by the muscles.
将沙漠蝗Schistocerca gregaria的普通抑制神经元（CI）和慢兴奋胫节神经元（SETi）所支配的慢肌135cd，在高亲和力摄取条件下于[3H]GABA或[3H]谷氨酸中孵育。[3H]GABA在CI以及SETi神经末梢的神经胶质中积累；然而，它仅在CI的终末轴突中积累，而不在SETi的终末轴突中积累。神经胶质和CI终末轴突上方的银粒密度约为2个银粒/微米²。在[3H]谷氨酸中孵育后，CI终末轴突和SETi终末轴突上方的银粒密度约为4个银粒/微米²；两种神经末梢神经胶质上方的银粒密度约为17个银粒/微米²。在谷氨酸存在下孵育后肌肉相对较高的标记（3个银粒/微米²）归因于慢肌的高代谢需求。得出的结论是，CI终末轴突以及CI和SETi神经末梢的神经胶质中存在GABA的高亲和力摄取系统。然而，虽然慢肌135cd的CI和SETi神经末梢中的谷氨酸摄取与快速收缩爪肌的快速兴奋胫节神经元（FETi）神经末梢中谷氨酸的高亲和力摄取相当，但仅在两种神经末梢的神经胶质中证明了谷氨酸的高亲和力摄取。CI和SETi终末轴突中的高亲和力摄取可能被肌肉对谷氨酸广泛的低亲和力摄取所掩盖。
