Costa Jonas Henrique, da Costa Bruna Zucoloto, de Angelis Derlene Attili, Marsaioli Anita Jocelyne
Institute of Chemistry, State University of Campinas-UNICAMP, PO Box 6154, Campinas, SP, 13083-970, Brazil.
Division of Microbial Resources, Chemical, Biological and Agricultural Pluridisciplinary Research Center-CPQBA, State University of Campinas-UNICAMP, Campinas, SP, 13148-218, Brazil.
Appl Microbiol Biotechnol. 2017 Aug;101(15):6061-6070. doi: 10.1007/s00253-017-8389-z. Epub 2017 Jun 28.
High-throughput screening detected transaminases (TAs) and monoamine oxidases (MAOs) in fungi by applying a fluorogenic probe. Strains F026, F037, F041, F053, and F057 showed the highest enzymatic conversions (31, 60, 30, 40, and 32%, respectively) and where evaluated for their ability to transform piperidines. Strain F053 (Neopestalotiopsis sp. CBMAI 2030) revealed unusual enzymatic activity to deracemize 2-methyl-6-alkylpiperidines. Neopestalotiopsis sp. CBMAI 2030 was capable to convert 2-methyl-6-propylpiperidine, 2-methyl-6-butylpiperidine, and 2-methyl-6-pentylpiperidine in piperideine with 11, 14, and 24% conversion, respectively. The activity was enhanced by cultivating the fungus with 2-methyl-6-pentylpiperidine (38% conversion and 73% ee).
高通量筛选通过应用荧光探针检测真菌中的转氨酶（TAs）和单胺氧化酶（MAOs）。菌株F026、F037、F041、F053和F057表现出最高的酶促转化率（分别为31%、60%、30%、40%和32%），并对它们转化哌啶的能力进行了评估。菌株F053（新拟盘多毛孢属CBMAI 2030）显示出对2-甲基-6-烷基哌啶进行消旋化的异常酶活性。新拟盘多毛孢属CBMAI 2030能够分别将2-甲基-6-丙基哌啶、2-甲基-6-丁基哌啶和2-甲基-6-戊基哌啶转化为哌啶，转化率分别为11%、14%和24%。通过用2-甲基-6-戊基哌啶培养真菌，该活性得到增强（转化率为38%，对映体过量值为73%）。
