Bandaly Victor, Joubert Aurélie, Le Cann Pierre, Andres Yves
GEPEA-CNRS, UMR 6144, IMT Atlantique, 44000, Nantes, France.
EHESP, 35000, Rennes, France.
Food Environ Virol. 2017 Dec;9(4):464-472. doi: 10.1007/s12560-017-9310-8. Epub 2017 Jun 28.
One of the most important topics that occupy public health problems is the air quality. That is the reason why mechanical ventilation and air handling units (AHU) were imposed by the different governments in the collective or individual buildings. Many buildings create an artificial climate using heating, ventilation, and air-conditioning systems. Among the existing aerosols in the indoor air, we can distinguish the bioaerosol with biological nature such as bacteria, viruses, and fungi. Respiratory viral infections are a major public health issue because they are usually highly infective. We spend about 90% of our time in closed environments such as homes, workplaces, or transport. Some studies have shown that AHU contribute to the spread and transport of viral particles within buildings. The aim of this work is to study the characterization of viral bioaerosols in indoor environments and to understand the fate of mengovirus eukaryote RNA virus on glass fiber filter F7 used in AHU. In this study, a set-up close to reality of AHU system was used. The mengovirus aerosolized was characterized and measured with the electrical low pressure impact and the scanner mobility particle size and detected with RT-qPCR. The results about quantification and the level of infectivity of mengovirus on the filter and in the biosampler showed that mengovirus can pass through the filter and remain infectious upstream and downstream the system. Regarding the virus infectivity on the filter under a constant air flow, mengovirus was remained infectious during 10 h after aerosolization.
空气质量是占据公共卫生问题的最重要主题之一。这就是不同政府在集体或个人建筑中强制要求使用机械通风和空气处理机组（AHU）的原因。许多建筑使用供暖、通风和空调系统营造人工气候。在室内空气中现有的气溶胶中，我们可以区分出具有生物性质的生物气溶胶，如细菌、病毒和真菌。呼吸道病毒感染是一个主要的公共卫生问题，因为它们通常具有高度传染性。我们大约90%的时间都在封闭环境中度过，如家庭、工作场所或交通工具中。一些研究表明，空气处理机组会导致病毒颗粒在建筑物内传播和扩散。这项工作的目的是研究室内环境中病毒生物气溶胶的特征，并了解脑心肌炎病毒（一种真核RNA病毒）在空气处理机组中使用的F7玻璃纤维过滤器上的归宿。在本研究中，使用了一个接近实际空气处理机组系统的装置。对雾化的脑心肌炎病毒进行了表征和测量，采用了电低压冲击法和扫描迁移率粒径分析法，并通过逆转录定量聚合酶链反应（RT-qPCR）进行检测。关于脑心肌炎病毒在过滤器上以及生物采样器中的定量和感染性水平的结果表明，脑心肌炎病毒可以穿过过滤器，并在系统的上游和下游保持感染性。关于在恒定气流下过滤器上的病毒感染性，脑心肌炎病毒在雾化后10小时内仍具有感染性。
