Bandaly Victor, Joubert Aurélie, Andres Yves, Le Cann Pierre
1GEPEA-CNRS, UMR 6144, IMT Atlantique, 44000 Nantes, France.
2EHESP, 35000 Rennes, France.
Aerobiologia (Bologna). 2019;35(2):357-366. doi: 10.1007/s10453-019-09565-3. Epub 2019 Feb 4.
Viral aerosols can lead to respiratory viral infections with high infectivity. About 90% of people's time is spent in closed environments. A few studies have pointed out that the ventilation systems in air handling units (AHUs) that treat and transmit a new synthetic clean and conditioned environment can also spread and transport viral particles in buildings. The aim of this work is to study the characterization of adenovirus, a DNA non-enveloped respiratory virus, on the F7 fiberglass filter used in AHUs. In this study, an experimental setup simulating an AHU was used. The SYBR QPCR, Electrical Low-Pressure Impactor (ELPI™) and Scanning Mobility Particle Sizer (SMPS™) were used to detect, measure and characterize the aerosolized adenovirus solution. The characterization results showed that the nebulized adenovirus could be aerosolized in different forms associated or not with cell debris and proteins. The quantification and level of infectivity of adenovirus demonstrated that viruses passed through filters and remained infectious up- and downstream of the system during the 25 min of aerosolization. This study showed that AHUs should be considered an indoor source of viral contamination.
病毒气溶胶可导致具有高传染性的呼吸道病毒感染。约90%的人的时间是在封闭环境中度过的。一些研究指出，处理和传输新型合成清洁及调节环境的空气处理机组（AHU）中的通风系统也可在建筑物内传播和输送病毒颗粒。这项工作的目的是研究腺病毒（一种DNA无包膜呼吸道病毒）在AHU中使用的F7玻璃纤维过滤器上的特性。在本研究中，使用了模拟AHU的实验装置。采用SYBR QPCR、电低压冲击器（ELPI™）和扫描迁移率粒径分析仪（SMPS™）对雾化腺病毒溶液进行检测、测量和表征。表征结果表明，雾化的腺病毒可以以与细胞碎片和蛋白质相关或不相关的不同形式被雾化。腺病毒的定量和感染性水平表明，在雾化25分钟期间，病毒通过过滤器并在系统的上下游保持感染性。这项研究表明，AHU应被视为室内病毒污染的一个来源。
