Department of Biomedical Laboratory Science and Management, Vidyasagar University, Midnapore, West Bengal, 721102, India.
Clinical Nutrition and Dietetics Division (Funded under UGC Innovative Programme), Vidyasagar University, Midnapore, West Bengal, 721102, India.
Biol Trace Elem Res. 2018 Mar;182(1):78-90. doi: 10.1007/s12011-017-1077-0. Epub 2017 Jun 28.
Continuation of prolonged treatment against arsenicosis with conventional chelating therapy is a global challenge. The present study was intended to evaluate the defensive effect of arjunolic acid against arsenic-induced oxidative stress and female reproductive dysfunction. Wistar strain adult female rats were given sodium arsenite (10 mg/kg body weight) in combination with arjunolic acid (10 mg/kg body weight) orally for two estrous cycles. Electrozymographic analysis explored that arjunolic acid co-treatment counteracted As-induced ROS production in uterine tissue by stimulating the activities of endogenous enzymatic antioxidants. Arjunolic acid was able to enhance the protection against mutagenic uterine DNA breakage, necrosis, and ovarian-uterine tissue damages in arsenicated rats by improving the ovarian steroidogenesis. The mechanisms might be coupled with the augmentation of antioxidant defense system, partly through the elimination of arsenic with the involvement of S-adenosyl methionine pool where circulating levels of vitamin B, folic acid, and homocysteine play critical roles as evidenced from our present investigation.
长期使用传统螯合疗法治疗砷中毒是一个全球性的挑战。本研究旨在评估熊果酸对砷诱导的氧化应激和女性生殖功能障碍的防御作用。Wistar 品系成年雌性大鼠连续两个发情周期经口给予亚砷酸钠（10mg/kg 体重）和熊果酸（10mg/kg 体重）。酶谱分析表明，熊果酸通过刺激内源性酶抗氧化剂的活性，拮抗 As 诱导的子宫组织 ROS 产生。熊果酸能够通过改善卵巢甾体生成，增强对砷中毒大鼠的子宫 DNA 断裂、坏死和卵巢-子宫组织损伤的保护作用。其机制可能与抗氧化防御系统的增强有关，部分机制可能与 S-腺苷甲硫氨酸池有关，其中循环维生素 B、叶酸和同型半胱氨酸水平起着关键作用，这从我们目前的研究中可以得到证明。
