Perveen Hasina, Chattopadhyay Sandip, Maity Moulima, Dash Moumita, Islam Syed Sirajul
Department of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics Division, UGC Innovative Department, Vidyasagar University, Midnapore, West Bengal, India.
Department of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics Division, UGC Innovative Department, Vidyasagar University, Midnapore-721102, West Bengal, India.
J Basic Clin Physiol Pharmacol. 2019 Jun 13;30(4):jbcpp-2017-0179. doi: 10.1515/jbcpp-2017-0179.
Background Curcumin is extensively used as a therapeutic intervention for treating several ailments. The antioxidant curcumin has an anti-inflammatory and chelating property with arsenic to exhibit a strong therapeutic effect on reproductive organs. This study was undertaken to describe the protective effect of noninvasive administration of curcumin against sodium-arsenite-mediated uterine hazards in female Wistar rats. Methods Twenty-four female Wistar rats were randomly divided into four groups. The treatment was continued for 8 days and given orally sodium arsenite (10 mg/kg body weight) in combination with curcumin (20 mg/kg body weight). Results Our evaluation revealed that 8 days of sodium arsenite (10 mg/kg body weight) treatment reduced the activities of the uterine enzymatic antioxidants superoxide dismutase, catalase, and peroxidase. Blood levels of vitamin B12 and folic acid decreased followed by an increased serum lactate dehydrogenase, homocysteine level, and hepatic metallothionein-1 in arsenic-treated rats. Necrosis of uterine tissue along with the disruption of ovarian steroidogenesis was marked in arsenic-treated rats with an upregulation of uterine NF-κB and IL-6 along with a raised level of serum TNF-α. Oral administration of curcumin (20 mg/kg body weight/day) in arsenic-treated rats significantly reinstated these alterations of the antioxidant system followed by an improvement of ovarian steroidogenesis and the circulating level of B12 and folate along with the downregulation of serum homocysteine, metallothionein-1, and cytokines. Conclusions The findings of this study clearly and strongly elucidated that arsenic-induced oxidative stress in uterus is linked to an alteration of inflammation-signaling biomarkers and these have been protected through the co-administration of curcumin due to its anti-inflammatory, free radical scavenging, and antioxidant activity by the possible regulation of an S-adenosine methionine pool.
姜黄素被广泛用作治疗多种疾病的治疗性干预措施。抗氧化剂姜黄素具有抗炎和与砷螯合的特性，对生殖器官具有强大的治疗作用。本研究旨在描述无创给予姜黄素对雌性Wistar大鼠亚砷酸钠介导的子宫损伤的保护作用。
将24只雌性Wistar大鼠随机分为四组。治疗持续8天，口服给予亚砷酸钠（10毫克/千克体重）与姜黄素（20毫克/千克体重）的组合。
我们的评估显示，8天的亚砷酸钠（10毫克/千克体重）治疗降低了子宫酶促抗氧化剂超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性。砷处理大鼠的血液维生素B12和叶酸水平降低，随后血清乳酸脱氢酶、同型半胱氨酸水平和肝脏金属硫蛋白-1升高。砷处理大鼠出现子宫组织坏死以及卵巢类固醇生成紊乱，子宫NF-κB和IL-6上调，血清TNF-α水平升高。在砷处理大鼠中口服给予姜黄素（20毫克/千克体重/天）显著恢复了抗氧化系统的这些改变，随后卵巢类固醇生成得到改善，B12和叶酸的循环水平提高，同时血清同型半胱氨酸、金属硫蛋白-1和细胞因子下调。
本研究结果清楚且有力地阐明，砷诱导的子宫氧化应激与炎症信号生物标志物的改变有关，通过联合给予姜黄素对这些改变起到了保护作用，这可能是由于姜黄素具有抗炎、自由基清除和抗氧化活性，通过调节S-腺苷甲硫氨酸池实现。
