Sicard Renaud, Landgraf Ralf
Vivex powered by UMTB, Miami, FL, USA.
Department of Biochemistry and Molecular Biology, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, 33136, FL, USA.
Methods Mol Biol. 2017;1609:57-63. doi: 10.1007/978-1-4939-6996-8_7.
High-performance thin-layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures. Here, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analyses for structural determination.