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High-Performance Chromatographic Separation of Cerebrosides.

作者信息

Sicard Renaud, Landgraf Ralf

机构信息

Vivex powered by UMTB, Miami, FL, USA.

Department of Biochemistry and Molecular Biology, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, 33136, FL, USA.

出版信息

Methods Mol Biol. 2017;1609:57-63. doi: 10.1007/978-1-4939-6996-8_7.

Abstract

High-performance thin-layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures. Here, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analyses for structural determination.

摘要

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