Ma Justin, Arbelo Ulises, Guerra Yenifer, Aribindi Katyayini, Bhattacharya Sanjoy K, Pelaez Daniel
Department of Ophthalmology, Miller School of Medicine, Dr. Nasser Al-Rashid Orbital Vision Research Center, Bascom Palmer Eye Institute, University of Miami, 1638 NW, 10th Ave., Miami, FL, 33136, USA.
Methods Mol Biol. 2017;1609:141-147. doi: 10.1007/978-1-4939-6996-8_13.
Electrospray ionization mass spectrometry has revolutionized the way lipids are studied. In this work, we present a tutorial for analyzing class-specific lipid spectra obtained from a triple quadrupole mass spectrometer. The open-source software MZmine 2.21 is used, coupled with LIPID MAPS databases. Here, we describe the steps for lipid identification, ratiometric quantification, and briefly address the differences to the analyses when using direct infusion versus tandem liquid chromatography-mass spectrometry (LC-MS). We also provide a tutorial and equations for quantification of lipid amounts using synthetic lipid standards and normalization to a protein amount.
电喷雾电离质谱法彻底改变了脂质的研究方式。在这项工作中，我们提供了一个教程，用于分析从三重四极杆质谱仪获得的特定类别脂质光谱。使用了开源软件MZmine 2.21，并结合脂质代谢物和途径策略（LIPID MAPS）数据库。在此，我们描述了脂质鉴定、比例定量的步骤，并简要阐述了与使用直接进样和串联液相色谱 - 质谱联用（LC-MS）分析时的差异。我们还提供了一个教程以及使用合成脂质标准品定量脂质含量并将其归一化为蛋白质含量的方程式。
