College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University , 94 Weijin Road, Tianjin 300071, China.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , 94 Weijin Road, Tianjin 300071, China.
Anal Chem. 2017 Jul 18;89(14):7277-7281. doi: 10.1021/acs.analchem.7b01348. Epub 2017 Jul 5.
We show a theranostic nanoplatform for messenger RNA (mRNA) triggered enhanced fluorescence imaging guided therapy. Catalytic hairpin assembly (CHA) and gold nanorods (AuNRs) are employed to fabricate the theranostic nanoplatform. Two hairpin DNAs and Cy5 labeled duplex DNA are integrated into the CHA for mRNA triggered fluorescence signal amplification via hybridization and displacement with mRNA. The AuNRs act both as the fluorescence quencher and the photothermal therapy (PTT) agent. The nanoplatform not only enables sensitive and specific imaging of target mRNA in living cells and good differentiating of the survivin mRNA expression levels in different cell lines but also offers excellent photothermal conversion efficiency for PTT. The developed nanoplatform has great potential for sensitive and specific intracellular mRNA imaging guided PTT.
我们展示了一种用于信使 RNA（mRNA）触发的增强荧光成像引导治疗的治疗性纳米平台。催化发夹组装（CHA）和金纳米棒（AuNRs）被用于制造治疗性纳米平台。两个发夹 DNA 和 Cy5 标记的双链 DNA 被整合到 CHA 中，通过与 mRNA 的杂交和置换，实现 mRNA 触发的荧光信号放大。AuNRs 既作为荧光猝灭剂，又作为光热治疗（PTT）剂。该纳米平台不仅能够在活细胞中灵敏且特异性地成像靶 mRNA，并且能够很好地区分不同细胞系中生存素 mRNA 表达水平，还具有优异的光热转换效率，用于 PTT。所开发的纳米平台在灵敏且特异性的细胞内 mRNA 成像引导 PTT 方面具有很大的潜力。
