Xu Yang, Niu Yong, Sun Fengjun, Yang Ying, Luo Wenbo, Wang Zhe
Department of Gynaecology & Obstetrics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Criminal Investigation Bureau, Ministry of Public Security, Beijing 100741, China.
Future Microbiol. 2017 Jun;12:573-584. doi: 10.2217/fmb-2016-0201. Epub 2017 Jun 29.
This study aims to characterize a multidrug-resistant (MDR) plasmid p12969-2 coexistent with the previously reported one p12969-DIM in clinical Pseudomonas putida.
MATERIALS & METHODS: The complete sequence of p12969-2 was determined using next-generation sequencing technology.
p12969-2 contains a 29.2 kb MDR region, which carries In127 harboring three resistance genes aadA2, qacED1 and sul1. The MDR region is derived from the connection of Tn5041D and Tn5045, which is facilitated by two copies of miniature inverted-repeat transposable element. Conclusion & future perspective: p12969-2 represents a novel lineage with the highest but limited nucleotide sequence similarity with the plasmid pGRT1 that does not carry any of the resistance genes. This is the first report of coexistence of two MDR plasmids in P. putida.
本研究旨在对临床恶臭假单胞菌中与先前报道的质粒p12969-DIM共存的多药耐药（MDR）质粒p12969-2进行特征分析。
使用下一代测序技术确定p12969-2的完整序列。
p12969-2包含一个29.2 kb的MDR区域，该区域携带In127，其中含有三个耐药基因aadA2、qacED1和sul1。该MDR区域源自Tn5041D和Tn5045的连接，这是由两个微型反向重复转座元件拷贝促成的。结论与未来展望：p12969-2代表了一个新的谱系，与不携带任何耐药基因的质粒pGRT1具有最高但有限的核苷酸序列相似性。这是关于恶臭假单胞菌中两个MDR质粒共存的首次报道。
