Chen Dingqiang, Yang Ling, Peters Brian M, Liu Junyan, Li Lin, Li Bing, Xu Zhenbo, Shirtliff Mark E
1 Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University , Guangzhou, People's Republic of China .
2 Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Science Center , Memphis, Tennessee.
Microb Drug Resist. 2019 Jan/Feb;25(1):1-7. doi: 10.1089/mdr.2018.0104. Epub 2018 Jul 23.
This study aimed at identification and characterization of a novel multidrug-resistant Pseudomonas putida strain Guangzhou-Ppu420 carrying two copies of qnrVC6 isolated from a hospital in Guangzhou, China, in 2012. Antimicrobial susceptibility was tested by Vitek2™ Automated Susceptibility System and Etest™ strips, and whole-genome sequencing facilitated analysis of its multidrug resistance. The genome has a length of 6,031,212 bp and an average G + C content of 62.01%. A total of 5,421 open reading frames were identified, including eight 5S rRNA, seven 16S rRNA, and seven 23S rRNA, and 76 tRNA genes. Importantly, two copies of qnrVC6 gene with three ISCR1 around, a bla carrying integron In528, a novel gcu173 carrying integron In1348, and six antibiotic resistance genes were identified. This is the first identification of two copies of the qnrVC6 gene in a single P. putida isolate and a class 1 integron In1348.
本研究旨在鉴定和表征一株新型多重耐药恶臭假单胞菌广州 - Ppu420，该菌株于2012年从中国广州一家医院分离得到，携带两个qnrVC6拷贝。通过Vitek2™自动药敏系统和Etest™试纸条检测抗菌药敏性，并利用全基因组测序对其多重耐药性进行分析。该基因组长度为6,031,212 bp，平均G + C含量为62.01%。共鉴定出5,421个开放阅读框，包括8个5S rRNA、7个16S rRNA和7个23S rRNA以及76个tRNA基因。重要的是，鉴定出两个带有三个ISCR1的qnrVC6基因拷贝、一个携带整合子In528的bla、一个携带整合子In1348的新型gcu173以及六个抗生素抗性基因。这是首次在单个恶臭假单胞菌分离株中鉴定出两个qnrVC6基因拷贝和1类整合子In1348。
