Yaffe M P, Ohta S, Schatz G
EMBO J. 1985 Aug;4(8):2069-74. doi: 10.1002/j.1460-2075.1985.tb03893.x.
We have previously described two yeast mutants which, at elevated temperature, stop growing and accumulate precursors to several imported mitochondrial proteins. We now show that one of these mutants (mas 1) is deficient in a matrix-located protease activity which cleaves the pre-sequences from mitochondrial precursor proteins. Isolated mas 1 mitochondria catalyze oxidative phosphorylation, exhibit respiratory control and import mitochondrial precursor polypeptides, but are defective in removing transient pre-sequences from imported precursors. The phenotype of the mas 1 mutant suggests that the matrix-located processing protease is essential for growth and for mitochondrial assembly.
我们之前描述过两种酵母突变体，在较高温度下，它们会停止生长并积累几种输入性线粒体蛋白的前体。我们现在表明，其中一种突变体（mas 1）缺乏一种位于线粒体基质的蛋白酶活性，这种活性可从线粒体前体蛋白上切割前序列。分离出的mas 1线粒体能够催化氧化磷酸化，表现出呼吸控制并输入线粒体前体多肽，但在从输入的前体中去除瞬时前序列方面存在缺陷。mas 1突变体的表型表明，位于线粒体基质的加工蛋白酶对于生长和线粒体组装至关重要。
